Cell Stress and Chaperones

, Volume 19, Issue 1, pp 145–153

Reaction of small heat-shock proteins to different kinds of cellular stress in cultured rat hippocampal neurons

Short Communication

DOI: 10.1007/s12192-013-0452-9

Cite this article as:
Bartelt-Kirbach, B. & Golenhofen, N. Cell Stress and Chaperones (2014) 19: 145. doi:10.1007/s12192-013-0452-9

Abstract

Upregulation of small heat-shock proteins (sHsps) in response to cellular stress is one mechanism to increase cell viability. We previously described that cultured rat hippocampal neurons express five of the 11 family members but only upregulate two of them (HspB1 and HspB5) at the protein level after heat stress. Since neurons have to cope with many other pathological conditions, we investigated in this study the expression of all five expressed sHsps on mRNA and protein level after sublethal sodium arsenite and oxidative and hyperosmotic stress. Under all three conditions, HspB1, HspB5, HspB6, and HspB8 but not HspB11 were consistently upregulated but showed differences in the time course of upregulation. The increase of sHsps always occurred earlier on mRNA level compared with protein levels. We conclude from our data that these four upregulated sHsps (HspB1, HspB5, HspB6, HspB8) act together in different proportions in the protection of neurons from various stress conditions.

Keywords

Stress response Neuroprotection Small heat-shock protein Hippocampal neuron 

Supplementary material

12192_2013_452_MOESM1_ESM.pdf (898 kb)
ESM 1(PDF 897 kb)

Copyright information

© Cell Stress Society International 2013

Authors and Affiliations

  1. 1.Institute of Anatomy and Cell BiologyUniversity of UlmUlmGermany

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