Original Paper

Cell Stress and Chaperones

, Volume 18, Issue 1, pp 25-33

First online:

Heat shock protein production and immunity and altered fetal development in diabetic pregnant rats

  • Felipe Hiroshi SaitoAffiliated withLaboratory of Experimental Research on Gynecology and Obstetrics, Department of Gynecology and Obstetrics, UNESP—Universidade Estadual Paulista, Botucatu Medical School
  • , Débora Cristina DamascenoAffiliated withLaboratory of Experimental Research on Gynecology and Obstetrics, Department of Gynecology and Obstetrics, UNESP—Universidade Estadual Paulista, Botucatu Medical School
  • , Bruna DallaquaAffiliated withLaboratory of Experimental Research on Gynecology and Obstetrics, Department of Gynecology and Obstetrics, UNESP—Universidade Estadual Paulista, Botucatu Medical School
  • , Iara Moreno LinharesAffiliated withDepartment of Gynecology, University of Sao Paulo Medical School and Hospital das Clínicas
  • , Marilza Vieira Cunha RudgeAffiliated withLaboratory of Experimental Research on Gynecology and Obstetrics, Department of Gynecology and Obstetrics, UNESP—Universidade Estadual Paulista, Botucatu Medical School
  • , Iracema De Mattos Paranhos CalderonAffiliated withLaboratory of Experimental Research on Gynecology and Obstetrics, Department of Gynecology and Obstetrics, UNESP—Universidade Estadual Paulista, Botucatu Medical School
  • , Steven S. WitkinAffiliated withLaboratory of Experimental Research on Gynecology and Obstetrics, Department of Gynecology and Obstetrics, UNESP—Universidade Estadual Paulista, Botucatu Medical SchoolDivision of Immunology and Infectious Diseases, Department of Obstetrics and Gynecology, Weill Cornell Medical CollegeDepartment of Obstetrics and Gynecology, Weill Cornell Medical College Email author 

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Abstract

We evaluated associations between the concentrations of heat shock proteins (hsp60 and hsp70) and their respective antibodies, alterations in maternal reproductive performance, and fetal malformations in pregnant rats with hyperglycemia. Mild diabetes (MD) or severe diabetes (SD) was induced in Sprague-Dawley rats prior to mating; non-treated non-diabetic rats (ND) served as controls. On day 21 of pregnancy, maternal blood was analyzed for hsp60 and hsp70 and their antibodies; and fetuses were weighed and analyzed for congenital malformations. Hsp and anti-hsp levels were correlated with blood glucose levels during gestation. There was a positive correlation between hsp60 and hsp70 levels and the total number of malformations (R = 0.5908, P = 0.0024; R = 0.4877, P = 0.0134, respectively) and the number of malformations per fetus (R = 0.6103, P = 0.0015; R = 0.4875, P = 0.0134, respectively). The anti-hsp60 IgG concentration was correlated with the number of malformations per fetus (R = 0.3887, P = 0.0451) and the anti-hsp70 IgG level correlated with the total number of malformations (R = 0.3999, P = 0.0387). Moreover, both hsp and anti-hsp antibodies showed negative correlations with fetal weight. The results suggest that there is a relationship between hsp60 and hsp70 levels and their respective antibodies and alterations in maternal reproductive performance and impaired fetal development and growth in pregnancies associated with diabetes.

Keywords

Diabetes Pregnancy Malformation Heat shock protein