Cell Stress and Chaperones

, Volume 15, Issue 4, pp 423–430

Stress response in tardigrades: differential gene expression of molecular chaperones

Authors

  • Andy Reuner
    • Zoology, Biological InstituteUniversität Stuttgart
  • Steffen Hengherr
    • Zoology, Biological InstituteUniversität Stuttgart
  • Brahim Mali
    • Molecular Biology and Functional GenomicsUniversity of Applied Sciences Wildau
  • Frank Förster
    • Department of Bioinformatics, Biocenter, Am HublandUniversity of Würzburg
  • Detlev Arndt
    • Developmental Biology UnitEuropean Molecular Biology Laboratory
  • Richard Reinhardt
    • MPI for Molecular Genetics
  • Thomas Dandekar
    • Department of Bioinformatics, Biocenter, Am HublandUniversity of Würzburg
  • Marcus Frohme
    • Molecular Biology and Functional GenomicsUniversity of Applied Sciences Wildau
  • Franz Brümmer
    • Zoology, Biological InstituteUniversität Stuttgart
    • Zoology, Biological InstituteUniversität Stuttgart
Original Paper

DOI: 10.1007/s12192-009-0158-1

Cite this article as:
Reuner, A., Hengherr, S., Mali, B. et al. Cell Stress and Chaperones (2010) 15: 423. doi:10.1007/s12192-009-0158-1

Abstract

Semi-terrestrial tardigrades exhibit a remarkable tolerance to desiccation by entering a state called anhydrobiosis. In this state, they show a strong resistance against several kinds of physical extremes. Because of the probable importance of stress proteins during the phases of dehydration and rehydration, the relative abundance of transcripts coding for two α-crystallin heat-shock proteins (Mt-sHsp17.2 and Mt-sHsp19.5), as well for the heat-shock proteins Mt-sHsp10, Mt-Hsp60, Mt-Hsp70 and Mt-Hsp90, were analysed in active and anhydrobiotic tardigrades of the species Milnesium tardigradum. They were also analysed in the transitional stage (I) of dehydration, the transitional stage (II) of rehydration and in heat-shocked specimens. A variable pattern of expression was detected, with most candidates being downregulated. Gene transcripts of one Mt-hsp70 isoform in the transitional stage I and Mt-hsp90 in the anhydrobiotic stage were significantly upregulated. A high gene expression (778.6-fold) was found for the small α-crystallin heat-shock protein gene Mt-sHsp17.2 after heat shock. We discuss the limited role of the stress-gene expression in the transitional stages between the active and anhydrobiotic tardigrades and other mechanisms which allow tardigrades to survive desiccation.

Keywords

Alpha-crystallin proteinAnhydrobiosisCryptobiosisHeat-shock proteinTardigradaMilnesium tardigradum

Copyright information

© Cell Stress Society International 2009