Cell Stress and Chaperones

, Volume 14, Issue 3, pp 253–263

Hsp72 chaperone function is dispensable for protection against stress-induced apoptosis

Original Paper

DOI: 10.1007/s12192-008-0079-4

Cite this article as:
Chow, A.M., Steel, R. & Anderson, R.L. Cell Stress and Chaperones (2009) 14: 253. doi:10.1007/s12192-008-0079-4


In addition to its role as a molecular chaperone, heat shock protein 72 (Hsp72) protects cells against a wide range of apoptosis inducing stresses. However, it is unclear if these two roles are functionally related or whether Hsp72 inhibits apoptosis by a mechanism independent of chaperone activity. The N-terminal adenosine triphosphatase domain, substrate-binding domain and the C-terminal EEVD regulatory motif of Hsp72 are all essential for chaperone activity. In this study, we show that Hsp72 mutants with a functional substrate-binding domain but lacking chaperone activity retain their ability to protect cells against apoptosis induced by heat and tumor necrosis factor alpha. In contrast, a deletion mutant lacking a functional substrate-binding domain has no protective capacity. The ability of the Hsp72 substrate-binding domain to inhibit apoptosis independent of the regulatory effects of the adenosine triphosphate-binding domain indicates that the inhibition of apoptosis may involve a stable binding interaction with a regulatory substrate rather than Hsp72 chaperone activity.


Hsp72Functional analysisApoptosisChaperone activity

Copyright information

© Cell Stress Society International 2008

Authors and Affiliations

  • Ari M. Chow
    • 1
  • Rohan Steel
    • 1
  • Robin L. Anderson
    • 1
    • 2
  1. 1.Peter MacCallum Cancer CentreEast MelbourneAustralia
  2. 2.Peter MacCallum Cancer CentreMelbourneAustralia