Original Article

International Journal of Hematology

, Volume 96, Issue 5, pp 600-610

First online:

Role of CD61+ cells in thrombocytopenia of dengue patients

  • Sansanee NoisakranAffiliated withDepartment of Pathology and Laboratory Medicine, Emory Vaccine Center, Emory University School of MedicineMedical Biotechnology Research Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency
  • , Nattawat OnlamoonAffiliated withCenter of Excellence for Flow Cytometry, Mahidol University
  • , Kovit PattanapanyasatAffiliated withCenter of Excellence for Flow Cytometry, Mahidol University
  • , Hui-Mien HsiaoAffiliated withDepartment of Pathology and Laboratory Medicine, Emory Vaccine Center, Emory University School of Medicine
  • , Pucharee SongprakhonAffiliated withCenter of Excellence for Flow Cytometry, Mahidol UniversityOffice for Research and Development, Mahidol University
  • , Nasikarn AngkasekwinaiAffiliated withDepartment of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University
  • , Kulkanya ChokephaibulkitAffiliated withDepartment of Pediatrics, Faculty of Medicine Siriraj Hospital, Mahidol University
  • , Francois VillingerAffiliated withDepartment of Pathology and Laboratory Medicine, Emory Vaccine Center, Emory University School of MedicineDivision of Pathology, Yerkes National Primate Research Center
  • , Aftab A. AnsariAffiliated withDepartment of Pathology and Laboratory Medicine, Emory Vaccine Center, Emory University School of Medicine
    • , Guey Chuen PerngAffiliated withDepartment of Pathology and Laboratory Medicine, Emory Vaccine Center, Emory University School of MedicineDepartment of Microbiology and Immunology, College of Medicine, NCKU Email author 

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Abstract

Although hematological disorders with salient features of thrombocytopenia have been well documented in dengue patients, the role of CD61-expressing platelets and the megakaryocytic cell lineage in the pathogenesis of dengue virus (DENV) infection remains largely unexplored. A prospective observational study was performed using blood samples and PBMCs from dengue-confirmed patients, as well as from rhesus monkeys (RM) experimentally infected with DENV. Immunohistochemical staining and FACS techniques were applied to evaluate the frequencies of CD61+ cells that contained DENV antigen. Highly enriched population of CD61+ cells was also isolated from acute DENV-infected RM and assayed for DENV RNA by quantitative RT-PCR. Results revealed that DENV antigen was found in small vesicles of varying size, and more frequently in anucleated cells associated with platelets in dengue patients. The DENV antigen-containing cells were CD61+ and appeared to share characteristics of megakaryocytes. Kinetic profiles of CD61+ cells from DENV-infected RM revealed a transient increase in CD61+CD62P+ cells early after DENV infection. DENV RNA in a highly enriched population of CD61+ cells from the infected RM was observed during acute stage. Our results indicate that virus containing CD61+ cells may be directly linked to the platelet dysfunction and low platelet count characteristics of dengue patients.

Keywords

Dengue Viremia Thrombocytopenia Fever Megakaryocytes DF DHF