International Journal of Hematology

, Volume 90, Issue 2, pp 261–269

Comparison of mesenchymal stem cells derived from arterial, venous, and Wharton’s jelly explants of human umbilical cord

  • Ikuo Ishige
  • Tokiko Nagamura-Inoue
  • Masaki J. Honda
  • Ratanakanit Harnprasopwat
  • Michiko Kido
  • Mitsuhiro Sugimoto
  • Hiromitsu Nakauchi
  • Arinobu Tojo
Original Article

DOI: 10.1007/s12185-009-0377-3

Cite this article as:
Ishige, I., Nagamura-Inoue, T., Honda, M.J. et al. Int J Hematol (2009) 90: 261. doi:10.1007/s12185-009-0377-3

Abstract

We isolated mesenchymal stem cells (MSC) from arteries (UCA), veins (UCV), and Wharton’s jelly (UCWJ) of human umbilical cords (UC) and determined their relative capacities for sustained proliferation and multilineage differentiation. Individual UC components were dissected, diced into 1–2 mm³ fragments, and aligned in explant cultures from which migrating cells were isolated using trypsinization. Preparations from 13 UCs produced 13 UCWJ, 11 UCV, and 10 UCA cultures of fibroblast-like, spindle-shaped cells negative for CD31, CD34, CD45, CD271, and HLA-class II, but positive for CD13, CD29, CD44, CD73, CD90, CD105, and HLA-class I. UCV cells exhibited a significantly higher frequency of colony-forming units fibroblasts than did UCWJ and UCA cells. Individual MSCs could be selectively differentiated into osteoblasts, chondrocytes, and adipocytes. When compared for osteogenic potential, UCWJ cells were the least effective precursors, whereas UCA-derived cells developed alkaline phosphatase activity with or without an osteogenic stimulus. UC components, especially blood vessels, could provide a promising source of MSCs with important clinical applications.

Keywords

Umbilical cordMesenchymal stem cellsHumanWharton's jellyExplants

Copyright information

© The Japanese Society of Hematology 2009

Authors and Affiliations

  • Ikuo Ishige
    • 1
    • 2
  • Tokiko Nagamura-Inoue
    • 1
  • Masaki J. Honda
    • 3
  • Ratanakanit Harnprasopwat
    • 4
  • Michiko Kido
    • 5
  • Mitsuhiro Sugimoto
    • 5
  • Hiromitsu Nakauchi
    • 2
  • Arinobu Tojo
    • 1
    • 4
  1. 1.Department of Cell Processing and Transfusion, Institute of Medical ScienceUniversity of TokyoTokyoJapan
  2. 2.Division of Stem Cell Therapy, Center for Stem Cell and Regenerative Medicine, The Institute of Medical ScienceUniversity of TokyoTokyoJapan
  3. 3.Department of Anatomy, School of DentistryNihon UniversityTokyoJapan
  4. 4.Division of Molecular of Therapy, Center for Advanced Medical Research, The Institute of Medical ScienceUniversity of TokyoTokyoJapan
  5. 5.Department of ObstetricsJapanese Red Cross Medical CenterTokyoJapan