International Journal of Hematology

, Volume 88, Issue 2, pp 181–188

Megakaryopoiesis and platelet function in polycythemia vera and essential thrombocythemia patients with JAK2 V617F mutation

  • Norimichi Hattori
  • Kunihiko Fukuchi
  • Hidetoshi Nakashima
  • Takashi Maeda
  • Daisuke Adachi
  • Bungo Saito
  • Kouji Yanagisawa
  • Isao Matsuda
  • Tsuyoshi Nakamaki
  • Kunihide Gomi
  • Shigeru Tomoyasu
Original Article

DOI: 10.1007/s12185-008-0129-9

Cite this article as:
Hattori, N., Fukuchi, K., Nakashima, H. et al. Int J Hematol (2008) 88: 181. doi:10.1007/s12185-008-0129-9

Abstract

Patients with Ph chromosome negative myeloproliferative disease (Ph-MPD) have an increased risk of vascular complications. It remains controversial whether patients with the JAK2 V617F mutation (V617F) exhibit increased risk, while recent growing evidence has shown a critical role for V617F in clonal erythropoiesis in Ph-MPD. We studied 53 patients with Ph-MPD especially in relation to megakaryopoiesis, the thrombotic complications and the presence of V617F. Using novel mutation-specific PCR which is a highly sensitive PCR-based assay for detection of JAK2 mutated allele(s), we identified V617F in 38 Ph-MPD, which include 13 polycythemia vera (PV), 23 essential thrombocythemia (ET) and 2 chronic idiopatic myelofibrosis. The numbers of megakaryocytes were significantly increased in PV and ET patients with V617F, but the platelet counts were slightly lower. Although statistically not significant, the incidence of thrombotic events was higher in the group with V617F compared to in those without the mutation. Agonist-induced in vitro platelet aggregation and platelet adhesion were not affected by the presence of this mutation. Nonetheless, we found a hypercoagulable state in Ph-CMPD with V617F by employing whole blood thromboelastography. It suggests pre-thrombotic tendencies in CMPD are complex and JAK2 V617F mutation might have a role in vivo blood coagulation by altering not only the number, but function(s) of all three myeloid cells, including red blood cells, white blood cells and platelets in Ph-CMPD.

Keywords

JAK2 V617F Ph chromosome negative myeloproliferative disease Mutation-specific PCR Megakaryopoiesis Thromboelastography 

Copyright information

© The Japanese Society of Hematology 2008

Authors and Affiliations

  • Norimichi Hattori
    • 1
    • 2
  • Kunihiko Fukuchi
    • 2
  • Hidetoshi Nakashima
    • 1
    • 2
  • Takashi Maeda
    • 1
  • Daisuke Adachi
    • 1
  • Bungo Saito
    • 1
  • Kouji Yanagisawa
    • 1
  • Isao Matsuda
    • 1
  • Tsuyoshi Nakamaki
    • 1
  • Kunihide Gomi
    • 2
  • Shigeru Tomoyasu
    • 1
  1. 1.Department of HematologyShowa University School of MedicineTokyoJapan
  2. 2.Department of Clinical PathologyShowa University, School of MedicineTokyoJapan