International Journal of Hematology

, Volume 87, Issue 2, pp 189–194

Tumor necrosis factor-α enhances DMSO-induced differentiation of HL-60 cells through the activation of ERK/MAPK pathway

Authors

  • Hong-Nu Yu
    • Department of Biochemistry, Institute of Medical ScienceChonbuk National University Medical School
  • Young-Rae Lee
    • Department of Biochemistry, Institute of Medical ScienceChonbuk National University Medical School
  • Eun-Mi Noh
    • Department of Biochemistry, Institute of Medical ScienceChonbuk National University Medical School
  • Kyung-Sun Lee
    • Department of Biochemistry, Institute of Medical ScienceChonbuk National University Medical School
  • Eun-Kyung Song
    • Department of Microbiology and ImmunologyChonbuk National University Medical School
  • Myung-Kwan Han
    • Department of Microbiology and ImmunologyChonbuk National University Medical School
  • Yong-Chul Lee
    • Department of Internal Medicine, Research Center for Allergic Immune DiseasesChonbuk National University Medical School
  • Chang-Yeol Yim
    • Department of Internal Medicine, Research Center for Allergic Immune DiseasesChonbuk National University Medical School
  • Jinny Park
    • Division of Hematology/OncologyGachon University Medical Center
  • Byeong-Soo Kim
    • Department of Companion and Laboratory Animal ScienceKongju National University
  • Sung-Ho Lee
    • Department of Companion and Laboratory Animal ScienceKongju National University
  • Seung Jin Lee
    • Department of Pharmacy, College of PharmacyEwha Womans University
    • Department of Biochemistry, Institute of Medical ScienceChonbuk National University Medical School
Original Article

DOI: 10.1007/s12185-008-0037-z

Cite this article as:
Yu, H., Lee, Y., Noh, E. et al. Int J Hematol (2008) 87: 189. doi:10.1007/s12185-008-0037-z

Abstract

The differentiation of promyelocytic leukemic cells into mature cells is the major strategy for drug-based treatment of leukemia. Higher efficient methods to differentiate promyelocytic leukemic cells have been developed using various differentiation inducers including interferon-α, interleukin-4, tumor necrosis factor-α (TNF-α), and dimethyl sulfoxide (DMSO) as a single agent or in combination with each other. Here, we show that a combination of TNF-α with DMSO shows a synergic effect on HL-60 cell differentiation through the activation of ERK pathway. TNF-α enhanced CD11b expression and percent of cell population in the G1 phase induced by DMSO, which are hallmarks for HL-60 cell differentiation. Inhibition of ERK pathway abolished the synergic effect of TNF-α in combination with DMSO on HL-60 differentiation, but the inhibition NF-κB pathway did not. These results suggest that TNF-α synergistically increases DMSO-induced differentiation of HL-60 cells through the activation of ERK/MAPK-signaling pathway.

Keywords

DMSOTNF-αMAPKNF-κBLeukemic cell differentiation

Copyright information

© The Japanese Society of Hematology 2008