Abstract
The pomegranate (Punica granatum L.) is a fruit-bearing deciduous shrub. Various parts of this plant are used in the treatment of dyspepsia, bronchitis, hypotensive, throat inflammation, etc. and its nutritional value has well been accepted. In the present study, antioxidant and antimicrobial efficacy of different solvent fractions of P. granatum leaves was investigated. Fractionation was done sequentially in Soxhlet apparatus, using various solvents like petroleum ether, toluene, ethyl acetate, acetone, and water. Antioxidant activity was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay, superoxide anion radical scavenging assay, and reducing capacity assessment. Total phenol and flavonoid content was also measured. The antimicrobial activity was assessed by agar well diffusion method against some of the tested food borne, pathogenic, and skin disease causing microorganisms. Antiulcer activity of the P. granatum acetone fraction (PGA) was evaluated using ethanol-induced gastric ulcer model. The PGA had maximum phenol content and had higher antioxidant activity. The PGA showed better DPPH free radical and superoxide anion radical scavenging activity than that of the standards. The results demonstrated that acetone fraction had potent gastroprotective and antisecretory effects. This study suggests that sequential fractionation technique is an excellent technique of extracting bioactive components of this plant at least. This study also demonstrated that PGA can serve as a potential natural source for use in food, nutraceutical, and pharmaceutical industry.
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References
Abdel-Salam OM, Czimmer J, Debreceni A, Szolcsanyi J, Mozsik G (2001) J Physiol 95:105
Adiga S, Trivedi P, Ravichandra V, Deb D, Mehta F (2010) Asian Pac J Trop Med 3:687
Ahmed L, Martin-Diana AB, Rico D, Barry-Ryan C (2011) Food Chem 124:1451
Alper N, Acar J (2004) Die Nahrung 48:184
Amirghofran Z (2010) Iran J Immunol 7:65
Aruoma OI (2003) Mutat Res 523:9
Athukorala Y, Kim KN, Jeon YJ (2006) Food Chem Toxicol 44:1065
Awari DM, Mute VM, Thube BB (2009) J Pharm Res 2:182
Bajpai VK, Minkyun NA, Kang SC (2010) Food Chem Toxicol 48:1945
Brzozowski T, Konturek SJ, Kwiecien S, Pajdo R, Brzozowski I, Hahn EG (1998) J Clin Gastroenterol 27:125
Celik I, Temur A, Isik I (2009) Food Chem Toxicol 47:145
Chanda S, Dave R (2009) Afr J Microbiol Res 3:981
Chanda SV, Nagani KV (2010) Nat Sci 8:260
Chanda S, Baravalia Y, Kaneria M, Rakholiya K (2010a) Current research, technology and education topics in applied microbiology and microbial biotechnology. Formatex Research Center, Spain, 2:444
Chanda S, Dudhtra S, Kaneria M (2010b) Food Funct 1:308
Chang C, Yang M, Wen H, Chern J (2002) J Food Drug Anal 10:178
Das AK, Mandal SC, Banerjee SK, Sinha S, Das J, Saha BP, Pal M (1999) J Ethnopharmacol 68:205
Endo EH, Cortez DAG, Ueda-Nakamura T, Nakamura CV, Filho BPD (2010) Res Microbiol 161:534
Fang YZ, Yang S, Wu G (2002) Nutrition 18:872
Ganguly AK (1969) Experientia 25:1224
Gill NS, Dhawan S, Jain A, Arora R, Bail M (2012) Res J Med Plant 6:47
Gordon MF (1990) Food antioxidants. Elsevier, London, pp 1–18
Gulcin I, Huyut Z, Elmastas M, Aboul-Enein HY (2010) Arab J Chem 3:43
Halliwell B, Gutteridge JMC (1986) Free radicals in biology and medicine. Clarendon, Oxford
Hoogerwerf W, Pasricha PJ (2006) The pharmacological basis of therapeutics. Mc Graw Hill, New York, pp 967–981
Issa AY, Volate SR, Wargovich MJ (2006) J Food Comp Anal 19:405
Jurenka MTJ (2008) Altern Med Rev 13:128
Kalimuthu S, Rajesh P, Rajesh-Kannan V, Balamurugan B, Chandrasekar TM (2010) J Pharm Res 3:2779
Kaneria M, Baravalia Y, Vaghasiya Y, Chanda S (2009) Indian J Pharm Sci 71:406
Kaur G, Jabbar Z, Athar M, Alam AS (2006) Food Chem Toxicol 44:984
Ksouri R, Falleh HH, Megdiche W, Trabelsi N, Mhamdi B, Chaieb K, Bakrouf A, Magne C, Abdelly C (2009) Food Chem Toxicol 47:2083
Lansky EP, Newman RA (2007) J Ethnopharmacol 109:177
Lee CJ, Chen LG, Liang WL, Wang CC (2010) Food Chem 118:315
Lin J, Opoku AR, Geheeb-Keller M, Hutchings AD, Terblanche SE, Jager AK, Van Staden J (1999) J Ethnopharmacol 68:267
Liu J, Wang C, Wang Z, Zhang C, Lu S, Liu J (2011) Food Chem 126:261
Malik A, Mukhtar H (2006) Cell Cycle 5:371
Mc Donald S, Prenzler PD, Antolovich M, Robards K (2001) Food Chem 73:73
McCune LM, Johns T (2002) J Ethnopharmacol 82:197
Menezes SM, Cordeiro LN, Viana GS (2006) J Herb Pharmacother 6:79
Moure A, Cruz JM, Franco D, Dominguez JM, Sineiro J, Dominguez H, Nunez MJ, Parajo JC (2001) Food Chem 72:145
Nair R, Chanda S (2005) Indian J Pharm Sci 67:239
Nair R, Vaghaisya Y, Gogvani N, Solanki A, Baluja S, Chanda S (2008) Plant Arch 8:671
Nascimento GGF, Locatelli J, Freitas PC, Silva GL (2000) Braz J Microbiol 31:247
Naveen A (2010) J Pharm Res 3:1119
Parmar HS, Kar A (2007) Nutr Res 27:710
Patel C, Dadhaniya P, Hingorani L, Soni MG (2008) Food Chem Toxicol 46:2728
Perez C, Paul M, Bazerque P (1990) Acta Biol Med Exp 15:113
Qingming Y, Xianhui P, Weibao K, Hong Y, Yidan S, Li Z, Yanan Z, Yuling Y, Lan D, Guoan L (2010) Food Chem 118:84
Raj RK (1975) Indian J Physiol Pharmacol 19:47
Rajkumar V, Guha G, Kumar RA (2011) Food Chem Toxicol 49:363
Rakholiya K, Kaneria M, Chanda S (2011) J Med Plants Res 5:63
Reddy MK, Gupta SK, Jacob MR, Khan SI, Ferreira D (2007) Planta Med 73:461
Rice-Evans CA (1995) Free radical and oxidative stress: environments, drugs and food additives. Portland, Portland, pp 103–116
Robak J, Gryglewski RJ (1988) Biochem Pharmacol 37:837
Schlesier K, Harwat M, Bohm V, Bitsch R (2002) Free Radic Res 36:177
Sturgeon SR, Ronnenberg AG (2010) Nutr Rev 68:122
Su X, Sangster MY, D’Souza DH (2010) Foodborne Pathog Dis 7:1473
Sulaiman SF, Yusoff NA, Eldeen IM, Seow EM, Sajak AAB, Supriatno OKL (2011) J Food Comp Anal 24:1
Sun J, Chu YF, Wu X, Liu RH (2002) J Agric Food Chem 50:7449
Toklu HZ, Sehirli O, Ozyurt H, Mayadagli AA, Eksioglu-Demiralp E, Cetinel S, Sahin H, Yegen BC, Ulusoylu-Dumlu M, Gokmen V, Sener G (2009) J Radiat Res 50:345
Trease BE, Evans WC (1992) Text book of pharmacognosy, 13th edn. Bailliere, Tinall Ltd, London pp. 202–205
Wallace JL (1993) Can J Physiol Pharmacol 71:98
Westh H, Zinn CS, Rosdahl VT, Sarisa (2004) Microb Drug Res 10:169
Wiart C, Hannah A, Yassim M, Hamimah H, Sulaiman M (2004) J Ethnopharmacol 95:285
Wongwattanasathien O, Kangsadalampai K, Tongyonk L (2010) Food Chem Toxicol 48:1045
Wu CR, Lin WH, Hseu YC, Lien JC, Lin YT, Kuo TP, Ching H (2011) Food Chem 127:564
Zhu KX, Lian CX, Guo XN, Peng W, Zhou HM (2011) Food Chem 126:1122
Zou Y, Lu Y, Wei D (2004) J Agric Food Chem 52:5032
Acknowledgments
The authors thank Prof. S.P. Singh, Head of the Department of Biosciences, Saurashtra University, Rajkot, Gujarat, India for providing excellent research facilities. One of the author Mr. Mital Kaneria is thankful to the University Grants Commission, New Delhi, India for providing financial support as Junior Research Fellow.
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Kaneria, M.J., Chanda, S.V. The Effect of Sequential Fractionation Technique on the Various Efficacies of Pomegranate (Punica granatum L.). Food Anal. Methods 6, 164–175 (2013). https://doi.org/10.1007/s12161-012-9412-8
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DOI: https://doi.org/10.1007/s12161-012-9412-8