Food Analytical Methods

, Volume 5, Issue 4, pp 784–794

Analysis of Pork Adulteration in Commercial Burgers Targeting Porcine-Specific Mitochondrial Cytochrome B Gene by TaqMan Probe Real-Time Polymerase Chain Reaction

  • M. Eaqub Ali
  • U. Hashim
  • Th. Sabar Dhahi
  • S. Mustafa
  • Yaakob Bin Che Man
  • Md. Abdul Latif
Article

DOI: 10.1007/s12161-011-9311-4

Cite this article as:
Ali, M.E., Hashim, U., Dhahi, T.S. et al. Food Anal. Methods (2012) 5: 784. doi:10.1007/s12161-011-9311-4

Abstract

A TaqMan probe real-time polymerase chain reaction assay was developed for the determination of pork adulteration in commercial burgers. The assay combined porcine-specific primers and TaqMan probe for the selective amplification and detection of a 109-bp fragment of swine cytochrome b (cytb) gene. Specificity test with 10 ng DNA of 11 different meat-providing animal and fish species yielded a quantification cycle (Cq) of 15.5 ± 0.20 for the pork and negative results for the others in a 40-cycle reaction with a change of analysts and sources. Analysis of beef burger formulations with spiked pork showed the assay can determine 100–0.01% contaminated pork with a PCR efficiency (E) of 93.8% and a correlation coefficient (R2) of 0.991. A plot of actual value against real-time PCR-predicted value also yielded a good linear regression, R2 0.998, and small root mean square error of calibration, RMSEC 0.42. A strong correlation was found between the partial least square (PLS)-predicted values and real-time PCR-determined values. The accuracy of the method was ≥90% in all determinations of the standard set. Residual analysis also revealed a high precision in all determinations. Finally, a random analysis of 10 ng DNA of commercial burgers from pork, beef, chicken, mutton, and chevon yielded a Cq of 15.56 ± 0.22 to 16.24 ± 0.35 from pork burgers, and negative results from the others, showing the suitability of the assay to determine pork in commercial burgers with a high accuracy and precision.

Keywords

Burger formulationDouble quenched TaqMan probeHalal and Kosher foodsPLS model

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • M. Eaqub Ali
    • 1
  • U. Hashim
    • 1
  • Th. Sabar Dhahi
    • 1
  • S. Mustafa
    • 2
  • Yaakob Bin Che Man
    • 2
  • Md. Abdul Latif
    • 3
  1. 1.Institute of Nano Electronic EngineeringUniversiti Malaysia PerlisKangarMalaysia
  2. 2.Halal Products Research InstituteUniversiti Putra MalaysiaUPM SerdangMalaysia
  3. 3.Department of Crop Science, Faculty of AgricultureUniversiti Putra MalaysiaUPM SerdangMalaysia