Biomass Production with Willow and Poplar Short Rotation Coppices on Sensitive Areas—the Impact on Nitrate Leaching and Groundwater Recharge in a Drinking Water Catchment near Hanover, Germany
- First Online:
- Cite this article as:
- Schmidt-Walter, P. & Lamersdorf, N.P. Bioenerg. Res. (2012) 5: 546. doi:10.1007/s12155-012-9237-8
- 1.3k Downloads
In a lowland drinking water catchment area, nitrate leaching as well as groundwater recharge (GWR) was investigated in willow and poplar short rotation coppice (SRC) plantations of different ages, soil preparation measures prior to planting and harvesting intervals. Significantly increased nitrate concentrations of 16.6 ± 1.6 mg NO3-N L−1 were measured in winter/spring 2010 on a poplar site, established in 2009 after deep plowing (90 cm) but then, subsequently decreased strongly to below 2 mg NO3-N L−1 in spring 2011. The fallow ground reference plot showed nitrate concentrations consistently below 1 mg L−1 and estimated annual seepage output loss was only 1.36 ± 1.1 kg ha−1 a−1. Leaching loss from a neighboring willow plot from 2005 was 14.3 ± 6.6 kg NO3-N ha−1 during spring 2010 but decreased to 2.0 ± 1.5 kg NO3-N ha−1 during the subsequent drainage period. A second willow plot, not harvested since its establishment in 1994, showed continuously higher nitrate concentrations (10.2 ± 1.7 NO3-N L−1), while a neighboring poplar plot, twice harvested since 1994 showed significantly reduced nitrate concentrations. Water balance simulations, referenced by soil water tension and throughfall measurements, showed that at 655 mm annual rainfall, GWR from the reference plot (300 mm a−1) was reduced by 40 % (to 180 mm a−1) on the 2005 willow stand, mainly due to doubled rainfall interception losses. However, transpiration was limited by low soil water storage capacities, which in turn led to a moderate impact on GWR. We conclude that well-managed SRC on sensitive areas can prevent nitrate leaching, while impacts on GWR may be mitigated by management options.
KeywordsSRCGroundwater qualitySandy soilEvapotranspirationLeaf area index
To combat climate change and improve security of energy supply, bioenergy derived from forestry and agriculture plays a key role in the European Union (EU). Bioenergy production has almost doubled in production in the last 15 years and currently supplies 7 % of the total EU primary energy . According to the binding targets set by the EU Renewable Energy Directive (RED), all Member States should strive to a 20 % share of renewable energy by 2020. Furthermore, it is required that EU member states achieve at least a 10 % share of renewable energy (biofuel) of the total gasoline and diesel consumed in the transport sector by the year 2020 .
Bioenergy crops from agriculture provide the largest potential to fulfill those EU targets. An assessment made by the European Environment Agency found that about 85 % of the potential bioenergy supply can be produced by only seven member states (Spain, France, Germany, Italy, UK, Lithuania and Poland; ). To achieve these goals, approximately 17.5 million ha of land will have to be dedicated to the production of energy crops by 2020 . Thus, an additional pressure on farmland biodiversity as well as on soil and water resources can be expected in biofuel production regions in the EU.
In Germany, approximately 2.3 million ha or 19 % of the crop land is already being used for the production of renewable raw materials . Compared to 2001, the area has almost tripled and in 2011 the largest proportion of about 2 million ha fell to the energy plant production with a share of 46 % for biodiesel (mainly canola), 41 % for biogas (mainly maize) and 13 % for the production of bioethanol. Although perennial energy crops like short rotation coppices (SRC) with fast-growing trees have played only a minor role in bioenergy production, the total cultivated area for SRC increased from about 4,000 ha in 2010 to about 5,000 ha in just 1 year .
Nevertheless, SRC may provide unique ecological services that warrant consideration. As a result of lower fertilizer requirements as well as a higher N-use efficiency due to effective N-recycling, SRC emit 40 to >99 % less N than conventional annual crops. Furthermore, SRC have the potential to sequester additional carbon (0.44 Mg soil C ha−1 year−1) in soils if established on former cropland . According to Djomo et al. , SRC yielded about 14–86 times more energy than coal per unit of fossil energy input and greenhouse gas (GHG) emissions were 9–161 times lower than those of coal. Consequently, SRC provide an opportunity to reduce dependency on fossil fuels and to mitigate GHG emissions. Therefore, SRC should be part of an overall strategy for achieving the minimum target for GHG emissions reduction as required by the EU RED . Additionally, SRC may also increase agricultural income diversification, enhance biodiversity, and reduce nutrient losses to the groundwater .
However, as the area requirements for bioenergy feedstock production increases, the pressure on marginal sites or fallow grounds with unfavorable site conditions may increase and SRC systems applied here may also have negative environmental impacts. Most importantly, it seems that SRC plots have to be optimally prepared by plowing to guarantee weed control during crop establishment [8–10]. Especially on fallow grounds, this may lead to an extra emission of CO2 and N20. According to Djomo et al. , these impacts depend on various factors such as the SRC cultivation practice, land management, site conditions, downstream processing and distribution routes. Furthermore, indirect impacts have to be considered. For instance, N2O emissions as a direct impact may be low on well-drained and well-aerated soils, but NO3 leaching may occur instead and contaminate adjacent water bodies [6, 11, 12].
Accordingly, the given study is focusing on such indirect emission effects, i.e., the risk of nitrate leaching during the establishment of SRC plantations, and the potential of nitrogen binding after the cultivation of SRC on fallow ground.
Our study site was located in the most important drinking water catchment area of the city of Hanover, Germany (“Fuhrberger Feld”). Here, much effort was spent by the water authorities during the last decades to keep the average seepage nitrate concentration on the catchment level below the legal drinking water threshold value of 10.3 mg NO3-N L−1. Part of these efforts were voluntary agreements with resident farmers to reduce fertilizer applications to a minimum, but also many fields were set aside to lie fallow.
The enhanced nitrate concentrations in the seepage output of Fuhrberger Feld are linked to the prevailing periglacial and sandy soils in the area and the historical land use. With the formerly widespread heath plaggen fertilization process, high amounts of carbon were brought into the sandy soils . In combination with originally high groundwater levels and pasture as predominant land use, high amounts of soil organic carbon accumulated in the topsoils of these areas. As long as these site conditions persisted (i.e., no changes in the water table or the grassland cover) those carbon stocks remained more or less stable. However, since the 1960s, a significant increase of the drinking water demand of the city of Hanover lowered the groundwater table considerably, with the result that the wet grassland fell dry. This lowering of the groundwater table and the subsequent transfer of grassland into intensively used arable land initiated a strong mineralization process, including the transfer of organic N to nitrate. According to model calculations of Springob et al.  and Springob and Kirchmann , it was estimated that it might take up to 100 years for the soils to achieve a new equilibrium under the present conditions. Under these conditions, Köhler et al.  concluded that the only way to reduce the N output to groundwater is to convert the arable land into forests or back into continuous grasslands, while setting aside the land will not reduce the risk of nitrate leaching in the long run. However, another promising land-use for fallow grounds to meet the requirements of groundwater protection might be the establishment of SRC with fast-growing trees like poplar or willow.
The desired positive effect of reducing nitrate leaching losses by SRC might come along with negative effects on groundwater quantity, as higher rates of transpiration and interception evaporation can be anticipated [17–24]. In a review , Dimitriou et al. summarized that groundwater recharge rates from SRC stands in general are expected to be lower when compared to arable fields or grassland in the same region. Moreover, there is indication that the amount of reduction beyond precipitation strongly depends on site-specific conditions like soil type, occurrence of drought periods during the growing season and management practices such as the harvesting interval.
Biomass production by SRC might thus conflict with the assigned land use purpose in Fuhrberger Feld, i.e., to provide and guarantee adequate amounts of good quality drinking water for the city of Hanover.
Within this context, the objectives of our study were to evaluate the impact of SRC cultivation on (1) nitrate leaching losses and (2) groundwater recharge, by giving initial insights into basic soil background conditions, seepage nitrate concentrations and water budgets of four SRC stands (two willow and two poplar SRCs) in comparison with a fallow ground reference site. The studied SRC stands differ in stand age, soil preparation measures and harvesting regimes. Water balance components are calculated by applying a soil vegetation atmosphere transport model (CoupModel ) to the reference site and a willow SRC established in 2005. The simulations are parameterized with results from field measurements; the model performance is checked by observed soil water tensions and stand precipitation. Finally, we estimate nitrate seepage output rates by combining simulated drainage flux with measured nitrate concentrations. The following questions will be addressed: Is a change in land use from fallow ground to SRC associated with increased N leaching rates? Which factors control N leaching rates? Under present site conditions, does a probable reduction in groundwater recharge interfere with the production of drinking water? How do site and vegetation characteristics affect the water balance and what management options do we have to mitigate a negative impact on groundwater recharge?
Material and Methods
Site and Research Plot Description
The Fuhrberger Feld drinking water catchment is located in northwest Germany, approximately 30 km north of the city of Hanover and has a size of 308 km2. Within this catchment area, research plots are located northwest of the village Fuhrberg (52° 36′ N, 9° 51′ E) in a level 2 drinking water sanctuary  at an elevation of 41 m asl. Average annual precipitation (1971–2000) is 670 mm, of which 46 % fall during the growing season (May–October) and the mean annual temperature is 9.2 °C.
Basic site and soil type background conditions of the research plots in the Fuhrberger Feld (SR/DR = single/double row; 2/0.8 × 0.6 m = 2 m between, 0.8 m within DR; 0.6 m within SR)
Soil treatment before planting
Soil type, soil horizon sequence, depth (cm)
Harvesting interventions until 2011
Deep-plowing up to 90 cm soil depth with a double-blade plowshare (i.e., transfer of the humic top layer (30 cm) to a depth of 30–60 cm)
Mixture of clone Maxa1–3
DR (2/0.8 × 0.8 m)b
R2 + E (30–60)
Conventional plowing up to 30 cm soil depth
2 (winter 2005 + spring 2011)
Row mixture of 18 clones
SR (0.5 × 2 m)b
1 (spring 2011)
DR (1.5/0.8 × 0.6 m)b
1 (spring 2010)
DR (1.5/0.9 × 0.5 m)b
Abandoned cropland since the early 1990s, i.e., today covered with a grass layer
Plaggenesch-Cambisol over Relictgley
Prior to SRC cultivation from cuttings, plots Ref, P09/90 and W05/90 were part of one single arable field, which was set aside for groundwater protection reasons in the early 1990s. The Ref plot is dominated by grasses and some scattered flowers, due to the long time of abandonment. Plots W05/90 and P09/90 were deeply plowed to a maximum soil depth of 90 cm. As a result, the Ah layer and its seedbank, as found on Ref, was buried at a depth of 30–60 cm (R2 + E horizon) and covered with sandy bedrock material (R1). The site preparation allowed willow cuttings a headstart over the competing grasslayer, which is now present in the field. In 2010, the tree mortality rate on W05/90 was 1.7 % . During the study period, several plots were harvested. In March 2010, W94/30 was coppiced for the first time since its establishment in 1994, in April 2011 W05/90 followed. P94/30 was cut in March 2011 for the second time after February 2006.
Collection of Soil Solution and Soil Samples
Field installations for soil solution sampling, the sampling process itself, inclusive storage, transport and pre-treatment of the soil solution before the laboratory analysis were in line with the ICP IM manual (2004) for soil water chemistry .
Accordingly, six suction lysimeters per plot (polyvinyl chloride (PVC) pipe, 95 × 2 cm, connected with a P-80 cup, 5 × 2 cm, CeramTec Ag, Marktredwitz, Germany) were installed in November 2009, below the main rooting zone in 100 cm soil depth. After predrilling with a slightly smaller auger than the suction cups, the lysimeters were pushed directly into the soil, without applying additional active filling material. Lysimeters were evenly distributed in and between the tree rows. The soil solution was gathered in evacuated (max. 0.6 bar) 1-L glass vials, each connected via buried PVC tubing to one suction lysimeter and placed in a buried cool box next to the lysimeter field. Following the ICP manual (2004), soil solution was sampled bi-weekly to monthly. For transport and storage, solution samples were transferred into 100-ml PVC bottles and immediately stored in dark and cool conditions with a maximum temperature of 4 °C. As samples should be analyzed for all major anions and cations and to avoid any analytical interference, no preservative was added prior to the analysis, which was done within the following month after field sampling. Due to relatively dry soil conditions between June and November 2010, no soil solution could be extracted during this time.
Soil samples for physical and chemical analysis were taken from one soil pit per field plot. From each horizon, three single samples were taken to determine chemical properties. On the research plots that were chosen for water balance simulations (Ref, W05/90), volume intact soil samples were taken using steel cylinders (100 cm³) to determine soil physical properties of the soil horizons. At W05/90, five replicate samples in soil depths of 5, 15, 25, 35, 65 and 100 cm were taken, to account for the heterogeneous soil profile caused by soil preparation measures. At the more homogenous profile of Ref, three replicate samples in depth 15, 45 and 100 cm depth were taken.
The pH was measured on dried (40 °C, >48 h) and sieved (≤2 mm) soil samples using a digital pH/conductivity meter at a soil to water ratio of 1:2.5 (WTW GmbH Weilheim, West Germany). Total organic carbon (Corg) and total nitrogen (Nt) from mineral soil samples was measured from dried (40 °C, >48 h) and grounded samples using a C-N analyzer, (CHN-O-Rapide, VarioEL, Elementar, Germany). Our detection limit for total N is ≤0.2 mg g−1 and for total C ≤0.1 mg g−1. The C/N ratio was calculated from the obtained Corg to Nt values.
The mineral N content (Nmin, NH4+ + NO3−) was detected after extraction with 0.5 M K2SO4. NH4+ and NO3− were determined by using continuous flow injection colorimetric (Cenco/Skalar Instruments, Breda, The Netherlands). NH4+ was determined using the Berthelot reaction method (Skalar Method 155-000), NO3− in the K2SO4 -extract as well as in the soil solution was determined using the copper–cadmium reduction method (Skalar Method 461-00). Total dissolved nitrogen (TDN) in the K2SO4 extract was analyzed by the given nitrate method after NH4+ and organic N compounds were converted by an alkaline persulphate and UV digestion to NO3−. Dissolved organic nitrogen (Norg) was computed as: Norg = TDN − (NH4+ N + NO3− N).
All soil water laboratory analyses were applied in line with the aforementioned ICP Manual (2004; here section 8, Data Quality Assurance and Management ) and soil solution nitrate analysis was cross-checked by the correlation of NO3-N + NH4-N to total N (R2 = 0.985). Furthermore, quality control of our laboratory analysis are regularly applied by the integration of internal standards, replicate measurements and the contribution to external ring analysis (e.g., , Lab Code A56  Lab No. 44).
Soil water retention characteristics were analyzed for the W05/90 and Ref using the soil cores placed on a pressure membrane apparatus. Volumetric water contents were determined at pressure heads of pF 1.0, pF 1.5, pF 1.8, pF 2.0, pF 2.3, pF 2.5, pF 3.0, pF 3.3, pF 3.5, pF 3.7 and pF 4.2.
The grain size distribution of the fine soil was determined gravimetrically after oxidization of organic carbon using H2O2, destruction of binding components using Na–dithionite, following the method of Atterberg .
A climate station was set up on an open field approximately 150 m from the W05/90 plot and 200 m from the Ref plot, to collect meteorological data to be used as input variables for our water balance simulations. Sensors were mounted on a 10 m tall mast and read out by a datalogger (Dl2e, Delta-T Devices). Data were collected every 5 s, then aggregated at 10-min intervals. We measured precipitation at 1 m height (tipping bucket 0.1 mm, Thies Clima, Göttingen Germany), air temperature, relative humidity (both HMP45D, Vaisala, Vantaa, Finland), global radiation (SP Lite, Kipp & Zonen, Delft, The Netherlands) at 2 m height and wind speed (cup anemometer, Thies Clima) at 10 m height. For the use as model input, the data were checked for plausibility and later aggregated to hourly values. Data gaps in the time series due to equipment failure were filled with values from two nearby monitoring stations run by the German Weather Service. Precipitation values originate from a station about 4 km west of the field plots, while wind speed, relative humidity, air temperature and global radiation were taken from a station 15 km southwest from the field plots. Relative humidity and windspeed were adjusted to our site conditions by scaling daily mean values using linear relationships between our measurements and station data.
In 2010, precipitation was 651 mm, with 351 mm falling during the growing season (May–October). The annual sum for 2011 was 662 mm, of which 409 mm fell during the growing season. In 2010, a drought occurred in June and July, followed by a very wet period in August and September. The year 2011 was characterized by a cool and moist summer and a very dry and warm autumn.
Measurements of Soil Water Tension and Stand Precipitation
Soil water tensions to evaluate the simulation model performance were measured on the Ref and W05/90 plots at depths of 30 cm (n = 3), 60 cm (n = 3) and 100 cm (n = 10), using tensiometers (ceramic: P-80, CeramTec Ag, Marktredwitz, Germany) equipped with pressure transducers (PCFA6D, Honeywell; Morristown, NJ, USA). Pressure heads at 100-cm depth were recorded in hourly intervals from December 2009 using dataloggers (DL2 and DL2e, Delta-T Devices, Cambridge, UK). Monitoring of shallower soil depths began in May 2010. The tensiometers at 100-cm depth were placed in two parallel transects with a distance of 1 m between and within transects, crossing plant rows with an angle of 45°. The probes were installed at an angle of 30° to the soil surface in order to prevent preferential water flowing down the shaft of the instrument. Data quality assessment of water tension time series was done following the protocol described in Wegehenkel (2005) . Average soil water tensions were excluded from the model performance evaluation for periods where the values of one or more tensiometers had to be rejected, i.e., because dry soil conditions beneath the measuring limit (−850 hPa).
Stand precipitation measurements were conducted on W05/90 during the vegetation period 2010 using two 4 m long gutters with a width of 0.16 m (0.65 m2) made from stainless steel. The gutters were mounted on an 80 cm high wooden rack, water was collected in barrels (30 L) that were emptied when necessary, though at minimum every second week.
On the plots chosen for water balance simulations (Ref, W05/90), important vegetation characteristics like leaf area per unit ground area (leaf area index, LAI), canopy height and vertical root distributions were surveyed for the use as model input. Information on vertical root distributions came from Punzet (2011, unpublished). Canopy height was measured after the growing seasons using a measuring rod. LAI was measured with a Sunscan light interception probe (SS1, Delta-T Devices, Cambridge, UK) at three dates per growing season. As recommended by the manual of the probe, the measurements were conducted on days with stable light conditions, either on bright and sunny days or on days with a uniform overcast sky. On each measuring campaign, 50 readings were taken on fixed transect points inside the canopy. In order to avoid boundary effects, all measuring points were more than three tree lengths away from the stand edges. Before each 10 readings, the probe was referenced by measuring incident radiation outside the canopy on an open field, as well three tree lengths away from the stand edges.
All soil properties and nitrate concentration data were checked to satisfy the conditions of normal distribution (Chi Quadrat test) homoscedasticity of residuals (Levene’s test) prior parametric testing. However, critical values (p ≤ 0.05) indicated a non-normal distribution and unequal variances of the soil properties data set. Thus, the non-parametric Kruskal–Wallis analysis of variance approach was used to find significant (p ≤ 0.05) differences between chemical parameters of plots for identical soil horizons (Table 4). Statistics on soil properties were applied using the software package STATISTICA, Version 9 (StatSoftGmbH, Hamburg, Germany).
Nitrate concentrations of the soil solution were analyzed using a linear mixed effects model , to account for the sample-point identity of measurements. The full model included the effect of the research plot, the drainage period (level “A”: spring 2010 and level “B”: winter/spring 2010/11) and their interaction effect. Sample point and sampling date were treated as random effects. Model comparisons were done using Akaike’s information criterion  and likelihood ratio tests , with the conclusion that the sampling date could be excluded from random effects. Diagnostic plots were used to check normality and homoscedasticity of residuals and proved no severe violation of assumptions. For identifying differences between nitrate concentration means on the plot and drainage period level, all orthogonal contrasts were specified. The original level of significance (α = 0.05) was adjusted to account for multiple comparisons of plots and periods. The analysis was conducted using the NLME package  provided by the statistical software R .
For evaluating the performance of the water balance simulation model, the coefficient of determination (R²) of a linear regression between simulated and observed values, the root mean square error (RMSE) and mean error (ME) were used as objective measures. The ME quantifies the mean absolute difference between simulated and observed values, RMSE is calculated as the square root of the mean squared difference between simulated and observed values.
The CoupModel (Version 3.0 ), formerly known as SOIL model, was used to estimate the components of the water balance of the Ref (grass cover) and W05/90 (willow canopy) plots. The CoupModel is a physical process model that simulates one-dimensional heat and water flows through a layered soil profile, which is covered with vegetation. It produces—after adjustment of soil and vegetation properties to site conditions—reliable estimates of evapotranspiration, groundwater recharge and other variables that are difficult to monitor in the field. In the past, it was successfully applied and verified on willow SRC stands [24, 38], crop production systems , forests [40, 41] and grass land sites . Soil water flows are calculated by solving Richard’s equation for saturated and unsaturated flow. This approach requires the hydraulic properties of the soil layers, that are described by the formulations of Brooks and Corey  (retention characteristics) and Mualem  (hydraulic conductivity). Richard’s equation allows for soil water sources and sinks, i.e., root water uptake driven by transpiration. Potential transpiration (Tp), interception evaporation (Ei) from wet plant surfaces and soil evaporation (Es) are calculated separately for one or more canopy layers and the soil surface using the Penman–Monteith combination equation . Actual transpiration as the sum of root water uptake from soil layers is calculated on the basis of potential transpiration, which is reduced by taking actual soil water availabilities, soil temperatures and root densities of the soil layers into account.
Simulation Setup and Parameterization
The simulations of the Ref and W05/90 plots were run with hourly resolution from January 2009 (initial soil water tension of all layers, −60 hPa) until the end of December 2011, driven by the meteorological input data set. The period of interest includes the years 2010 and 2011, for which daily output of water balance components and state variables were produced. For both simulations, soil profiles with 20 layers and a total depth of 2.55 m were defined. The thickness of the soil layers gradually increased from 5 cm in the uppermost 35 cm to 30 cm in the two deepest layers. Upper and lower boundary conditions were defined as flux boundaries with the upper boundary taking the stand precipitation into account. As lower boundary condition, a unit gradient gravitational water flow was setup, which in this study represents groundwater recharge. Capillary rise was not considered.
For the description of the physical and physiological properties of the willow canopy (i.e., stomata and aerodynamic resistance functions according to [46, 47]), we used the parameterisation of Persson and Lindroth  (Table 3). They simulated evapotranspiration rates of a willow stand on clay soil using the older version (SOIL) of the CoupModel and verified the model with measured stand evapotranspiration. R² ranged from 0.73 to 0.79, the model only slightly overestimated evapotranspiration during two growing seasons by 2 and 10 mm .
Soil hydraulic properties used in the water balance simulation for the plots Ref and W05/90
Air entry (hPa)
Wilting point (vol. %)
Residual water (vol%)
Matrix cond. (mm day−1)
Total cond. (mm day−1)
The observed root distribution was in good accordance to other reported observations  in SRC stands. Most fine roots (80 %) were found in the upper 60 cm of the soil profile. Below the R2 + E horizon, roots sporadically occurred down to a depth of 180 cm.
Adjusted CoupModel parameter values for the simulations of the W05/90 and Ref plots
Willow canopy W05/90
Grass layer Ref
Interception constant capacity (Ic)
Interception capacity per LAI (ILAI)
Interception surface resistance (rci)
Parameter in soil surface resistance function
Crit. threshold for water uptake reduction (Ψcrit)
Degree of root water uptake compensation from moist soil layers (RWUcomp)
Maximum stomata conductance (gmax)
Sensitivity of stomata conductance to VPD (b)
Sensitivity of stomata conductance to global short wave radiation (R0)
MJ m−2 day−1
Aerodynamic resistance (ra)
Leaf area index (LAI)
Critical air temperature for temperature sum calculation
Temperature sum to start leaf development
With respect to our measured stand precipitation and soil water tension data, adjustments of the original parameter sets [38, 42] had to be carried out, to obtain a better agreement between simulated and measured variables and thus a better estimation of the water balance components. Stand precipitation measurements on the willow plot indicated underestimated interception evaporation when using the canopy storage capacity parameters (Ic, ILAI) of a previous study , likely due to the higher temporal resolution (1 h) of our simulation. Adjustments of these parameters and the interception surface resistance (rci, Table 3) led to a better agreement between simulated and observed stand precipitation. Hydraulic conductivities derived from grain size distributions were adjusted considering the observed soil water tensions at field capacity during winter time. The RWUcomp parameter for water uptake compensation was used to improve the agreement between observed and simulated soil water tensions during the growing season.
As in the simulation of W05/90 plot, adjustments of the Ref parameter set included soil hydraulic conductivities and root water uptake compensation (RWUcomp). Additionally, the shape of the seasonal LAI development was adjusted.
Basic Soil Parameters
Mean (±SD) soil chemical background conditions of the SRC plots in the Fuhrberger Feld
Soil depth (cm)
pH (1 M KCl)
Nmin (NO3 + NH4) (mg/kg)
2.0 (0.2) a
0.2 (0.0) a
2.57 (0.81) a
5.7 (0.2) a
42.8 (8.2) a
2.5 (0.5) a
2.83 (0.86) b
71.7 (12.6) b
3.2 (0.6) b
6.72 (0.91) b
4.3 (0.1) b
1.9 (0.3) b
0.2 (0.0) b
Compared to conventional cropland sites of the region Nmin values are low. Higher Nmin values of the top soil—respectively the former top soil in 10–30 cm soil depth on the plowed P09/90 and W05/90 plots—are correlating with higher values of Norg. Furthermore, mean percentage of nitrate in total Nmin (NO3 + NH4) was not detectable in the 30–50 cm soil layer at the reference plot but also not in the most upper layer of the P09/90 plot—which in fact here is also the former deep layer, transferred by deep plowing to the top of the profile. As far as Nmin was detectable in deeper horizons at all SRC plots, nitrate was the dominant constituent. The pH (1 M KCl) values of all plots and soil layers range between 4.1 and 5.7, with a tendency towards higher values on the W05/90 plot. However, differences proved to be statistically significant in only one case (30–50 cm soil depth, W05/90versus Ref).
Nitrate Soil Solution Concentrations
In period A (spring 2010), there is a relatively clear sequence of the nitrate concentration levels between a mean of above 16 to below 1 mg NO3-N L−1 (P09/90 > W94/30 > W05/90 > P94/30 > Ref, Fig. 1). Concentrations of Ref and P94/30 thereby do not differ significantly from zero (Table 5a). In period B (winter/spring 2010/11; Table 5b), only the concentrations of W94/30 remain on the same high level of period A (no significant differences between periods). Nitrate concentrations of P94/30 in period B are as well at the very low concentration level of the reference plot, concentrations of P09/90 and W94/30 remain significantly higher than concentrations of Ref (Table 5). At the beginning of period B, nitrate concentrations of P09/90 started again at an elevated level but then strongly decreased to the level of the W05/90 plot. Finally, the estimated mean nitrate concentration of W05/90 in period B turned out to be significantly reduced compared to period A (Table 5a, b).
Significant differences of the estimated mean nitrate concentrations in period A were found for the comparisons Ref-P09/90, Ref-W94/30, P09/90-P94/30, and P09/90-W05/90 (Table 5a). In period B (Table 5b), the differences between W05/90 and P09/90 were not significant anymore, but the differences between W94/30 and P94/30 became significant.
However, as already described for the soil matrix, the spatial variability of the solute nitrate concentrations is high, especially when the concentration is low. In both sampling periods, the variation coefficient is between 100 and 200 % on the P94/30 plot, but is lower (around 14–65 %) when nitrate concentrations are enhanced (P09/90 and W94/30, both periods).
Simulated and Observed Pressure Heads
A similar seasonal pattern is revealed by tensiometry in summer 2011. After a series of heavy rain storms at the end of June, the soil in 100 cm depth on Ref is completely rewetted and remains relatively moist throughout the rest of the growing season. Potentials on W05/90 show only slight increases during that period. The soil stays relatively dry, though inside the measuring range until beginning of December 2011. Thus, considerable groundwater recharge cannot be expected before the end of the year.
Performance statistics for simulated soil water tensions and throughfall. Indicators for soil water tension performance are means for three (30–35 cm; 55–65 cm) respectively ten (95–105 cm) tensiometers
Soil water tension
Simulated Water Budgets
Simulated annual water balances of the Ref and W05/90 study plots, given in mm and as percentage of precipitation (% P)
Water balance component
Ref mm (% P)
W05/90 mm (% P)
Ref mm (% P)
W05/90 mm (% P)
∆ Water storagea
Similar to the seasonal variation in the soil water tensions, a clear pattern can be seen in the monthly sums of the water balance components of the study plots. Actual evapotranspiration (ETa) as the sum of transpiration (Ta), Ei and Es has the highest values during the growing season, whereas drainage from the soil profiles takes place mainly in winter. The transitions between these hydrological seasons are smooth, with a gradual shift towards ETa in spring and a more abrupt beginning of the drainage period in autumn or winter. Simulated monthly Ei and Ta from W05/90 are higher than from Ref (Fig. 3a); in summer, ETa generally is equal or even higher than precipitation. In July 2010, Ta is extraordinary low in both simulations, due to low amounts of precipitation in June and July and pronounced soil water deficits in the root zone.
The simulated groundwater recharge from W05/90 ceases during summer almost completely, while small amounts of drainage are formed on Ref throughout the whole observation period. On Ref, considerable monthly groundwater recharge rates are attained already in October 2010, while groundwater recharge from W05/90 does not start before December. In 2011, the winter drainage period did not start until the end of the simulation period, whereas seepage from Ref started to increase already in October.
The differences in seasonal water partitioning patterns between the simulated land use types are reflected in the annual sums of the water balance components. Especially annual Ei and Ta are higher from the willow stand (Table 7). During the simulation period, average Ei losses from W05/90 account for 25 % of precipitation, contrasted by 12 % on Ref. Simulated root water uptake on W05/90 is in both years about 60 mm higher.
The differences in annual evapotranspiration rates between the land use types result in large differences in the amount of water leaving the soil profiles and serving as groundwater recharge. On Ref, 345 mm drainage formed in the year 2010 (Table 7), which is more than half of the annual precipitation. In contrast to that, 189 mm (29 % of precipitation) groundwater recharge is formed on W05/90. In 2011, these differences are not as expressed, although the soil profile of W05/90 was not rewetted completely (Table 7) by the end of the simulation period. For the whole simulation period, average annual groundwater recharge from W05/90 (180 mm a−1) is approximately 40 % lower than groundwater recharge from Ref (300 mm a−1).
The German Biomass Research Center  has calculated that by 2020, there will be a net lack of about 270 PJ per year in the German energy and material related wood market. If this “wood gap” would be filled by the establishment of SRC plantations, it would result in an extra need of about 1.2 million ha . The current (2011) total cropland area in Germany is about 12 million ha, where approximately 2.3 million ha is used for bioenergy raw material production . As of today, SRC contribute to only approximately 5,000 ha , it may be unrealistic to fill the forecasted wood gap in Germany by using only SRC.
Thus, and in accordance with Fritsche et al. , the cultivation of bioenergy crops on natural or semi-natural land that is currently not under specific production, is expected to increase. However, in comparison with conventional crops for bioenergy production (e.g., canola, maize), SRC may even increase ecological services on the field as well as on the landscape level [52–56]. SRC may act as physical barriers in the formation of “arable deserts” and protect against soil erosion or act as riparian or groundwater buffer strips to protect soil and water qualities in the context of the Water Framework Directive 2006/118/EU .
Furthermore, the cultivation of biomass on unused degraded land or on abandoned farmland as applied in this study can be seen as a safeguard against negative indirect land use change effects, as described by Fritsche et al. .
In the given study, the general focus of the implemented SRC is to protect drinking water resources from nitrate pollution while simultaneously producing biomass feedstock on an extensive level, i.e., without any further input of N fertilizers or other chemicals. However, as drinking water is the most important product of land use, groundwater recharge rates have to be considered as well.
According to Gadgil et al. , the Fuhrberg recharge zone can thus be described as an “ecologically sensitive area” which is both, ecologically and economically important, but, vulnerable to even mild disturbances and hence demands careful management. In Fuhrberger Feld, reduced nitrate loads were achieved by setting aside arable cropland as demonstrated by the low nitrate leaching measured from the reference plot (Fig. 1).
Nevertheless, is it not clear how sustainable this approach is in the long-run. The C- and N-rich topsoil horizon is a potential source of nitrate leaching coming from mineralization pulses and accordingly may be vulnerable to any disturbances such as droughts, subsequent rewetting or even fire, all of which lead to a release of organically bound N sources. Such a disturbance effect can be followed on plot P09/90, where the site preparation measures (deep plowing) were followed by a distinct nitrate pulse in period A (Feb–May 2010). Enhanced amounts of organically bound N sources were made available and subsequently leached as nitrate to the groundwater.
However, nitrate leaching may also exhibit a high temporal variability, as evident from plot W05/90. Here, nitrate concentrations were in the range of the drinking water threshold value of 11.3 mg NO3-N L−1 (i.e., 50 mg NO3− L−1 ) during period A, but significantly decreased to the very low level of the reference plot in period B (Dec 2010 to Jun 2011). The same trend was evident for P94/30, though the starting nitrate concentrations were less pronounced (mean in period A, 3.3 mg L−1) but also exhibited considerable variability around the mean (±3.4 SD). As the nitrate concentrations in P94/30 were always clearly below the drinking water threshold, it can be concluded that SRC, at least in the long term, do not leach critical amounts of nitrate.
As indicated in Table 4, higher C and N contents as well as higher C/N ratios are present in the deeper soil horizons of the P94/30 plot. This finding might be regarded as a development towards more resilience against mineralization pulses and unwanted releases of C and N to the atmosphere and/or water sources.
As we do not know how the nitrate concentrations developed on W94/30 since establishment in 1994, we only can speculate: a few years after SRC establishment, the biological activity of the site will increase compared to the former cropland, due to the continuous input of leaf and root litter with no respective output losses of organic material by harvesting. In addition, different vertical soil structures (such as C and N accumulations in deeper soil layers) will develop. The most obvious indication of such development is that normally no leaf litter from the previous autumn can be found in spring and C and N is higher in horizons below the plowing depth of 30 cm.
Furthermore, N sources released by mineralization processes are protected from N-leaching as long as N uptake by the vegetation cover is balancing the N release. If plant growth stops for some reason—as it was evident on plot W94/30—but mineralization processes continue, nitrate leaching may occur. Harvesting may also stop the N uptake. But, since this is done normally during winter, when mineralization rates are low and the rootstock immediately re-sprouts once the weather gets warmer, nitrification pulses after harvest are not described so far, even in cases with additional N fertilization [59–61].
Comparable field data of nitrate leaching under SRC without additional N input manipulation (N fertilization, sewage sludge, waste water or compost additions) are rare. However, in one comparable study, Goodlass et al.  found enhanced nitrate leaching under a Salix viminals SRC, after the former canola field was plowed in winter and sprayed with herbicides in the following spring. Peak nitrate concentrations reached a maximum of 70 mg NO3-N L−1 in spring and were even enhanced to 134 mg NO3-N L−1 during the following autumn. Once the SRC was established, concentrations returned to a lower level (18 mg NO3-N L−1) and were only slightly affected by harvesting operations and annual applications of nitrogen during the first 3 years. The reference plot was an adjacent arable area where nitrate peaks ranged from 26 to 77 mg NO3-N L−1 with an average value of 54 mg NO3-N L−1 during the crop rotation. Thus it was concluded, that once established, the risk of nitrate leaching from SRC grown at recommended N inputs is small, especially when compared with the nitrate peaks in autumn, which are typical of arable rotations . Moreover, significant losses during establishment of stands would be offset by smaller losses during the productive phase, when compared to average nitrate losses from crop production systems .
Land Use-Specific Water Budgets
The water balance simulations for the Ref and W05/90 study plots revealed distinct differences in partitioning precipitation into Ei, Ta and groundwater recharge. The differences in water budget partitioning are not surprising and generally agree with the findings of Persson , who compared the water budgets of different vegetation types and found highest evapotranspiration rates for spruce and willow and lowest for grassland and barley. However, considering the whole observation period, groundwater recharge from the willow stand was reduced by approximately 40 % (120 mm a−1) compared to the reference site. This is comparable to values for deciduous forests at comparable sites , but groundwater recharge from W05/90 was higher than values reported for coniferous forests  located at Fuhrberger Feld. The reduction thus is smaller than the reduction reported by Persson  for comparable land uses and reflects the need for water balance studies including various sites and different meteorological conditions. Furthermore, studies about the water balance of willow SRC on sandy soils with low amounts of plant available soil water storage are scarce in literature and data covering the whole year and not only the growing season are even scarcer.
Groundwater recharge from the willow stand was considerably reduced in comparison with the reference plot. The main reason for this shift from groundwater recharge to evapotranspiration lies in doubled interception losses, which are caused by a closer coupling of SRC stands to the atmosphere . This implies that evapotranspiration rates are mainly controlled by atmospheric vapor pressure deficit and aerodynamic resistance and less by available radiation energy . Together with a higher interception storage capacity of the willow canopy, simulated interception was approximately 25 % of annual precipitation in both years. This amount lies between two extremes reported by Persson and Lindroth  (11 %) and Ettala  (31 %).
It is surprising that during both years, the willow stand intercepted the same amount of rainfall (170 mm, Table 7) at nearly the same annual sum of precipitation, despite the fact that the stand was harvested in spring 2011. Re-sprouting stands are in the first half of the growing season less well coupled to the atmosphere because of their low stand height . Additionally, leaf area development is delayed compared to a mature stand. This implies a lower canopy interception storage capacity and before canopy closure, a higher amount of precipitation directly reaches the ground. Thus, it is conceivable that the harvested willow stand intercepts relatively less rainfall than a mature stand, and the lack of differences between the years is due to a different temporal distribution of rainfall. In fact, a simulation scenario that assumes a mature instead of a re-sprouting willow stand under the climate conditions of 2011 yields interception losses increased by 25 mm. In turn, a re-sprouting stand under the 2010 climate conditions has 15 mm less interception evaporation per year.
Aside from interception evaporation, transpiration rates from the willow stand are as well higher than transpiration rates from the reference site. With a deeper rooting system, the willow stand draws water from a greater soil volume, thus more water is available for transpiration. In both years, annual transpiration from the willow stand was approximately 60 mm higher compared to the reference site. This amount complies well with the surplus of plant available soil water resources. Tensiometer time series (Fig. 2b) indicate that the investigated willow SRC is able to evapotranspirate all precipitation that falls during the growing season (also see ) and additionally develops pronounced soil water deficits. These soil water deficits—being about 60 mm higher compared to the reference plot—in turn reduce the groundwater recharge, as the soil needs to be rewetted before drainage can take place.
A consequence of the willows’ high water demand in combination with the relatively low soil water storage capacity of the sandy soils is the exposition to water stress during periods with low amounts of precipitation. This is exactly what the simulation suggested in July 2010, when transpiration collapsed (Fig. 3b) because of exhausted soil water supplies in the root zone. Typical reactions to water stress are leaf shedding [66, 67] and yield losses . Leaf shedding was actually observed on the study plot in July 2010. Yield was not monitored during or immediately after the drought, but mean annual dry mass production at the end of the year 2009 was approximately 5.7 Mg ha−1 . This value is relatively low for willow SRCs  and indicates that growth conditions are not optimal in the Fuhrberger Feld, likely due to repeated water shortage.
Higher amounts of plant available soil water, either due to a higher soil water storage capacity as found in loamy soils, a greater rooting depth or even direct access to groundwater help to bridge extended dry periods and in terms of yield lead to more robust SRC production systems. However, this increased robustness is at the expense of groundwater recharge, since the soil water storage has to be refilled before drainage can form. Therefore, it can be concluded, that on sites with low plant available soil water capacity and where roots have no access to the water table, a change in land use from fallow to SRC indeed will have a negative impact on groundwater recharge. But on such sites, this impact is, as long as SRCs do not have access to groundwater, moderate: The soil water storage capacity sets a minimum level for groundwater recharge, but also sets the maximum limit to yield.
N released by Nitrate Leaching
Cumulated drainage water fluxes and nitrate leaching loss (±SD) for the Ref and W05/90 study plots during sampling periods A (Feb 2010-May 2010) and B (Dec 2010 –June 2011), as well as the annual sum for 2010
Drainage flux (L m−2)
Nitrate leaching (kg ha−1)
1.08 ± 0.85
14.3 ± 6.55
0.42 ± 0.38
2.03 ± 1.46
1.36 ± 1.08
16.5 ± 7.95
In total, the W05/90 plot lost 16.5 kg NO3-N ha−1 a−1 in 2010 (Table 8), where 87 % of the losses happened during winter/spring 2010. Nitrate leaching from the reference plot (Ref) was less than a tenth (1.36 kg of N03-N ha−1 a−1) of the amount of the SRC plot. Eighty percent of the annual leaching from Ref occurred during winter and spring 2010.
Calculated nitrate leaching losses for the W05/90 plot are slightly higher than reported in previous studies [59, 61]. There, leaching rates were between zero and slightly less than 2 kg ha−1 a−1, despite long-term repeated annual nitrogen fertilization of more than 150 kg ha−1 a−1 . However, our rates were about 10 times lower than rates cited by Aronsson and Bergström  for the first year after establishment, when willow was cultivated in lysimeters, highly fertilized and irrigated on sandy soils (140 kg N03-N ha−1 a−1). Similar N loss with seepage of 90 kg N03-N ha−1 a−1 were measured by Dimitriou and Aronsson  from a lysimeter experiment, where irrigated willows in sandy soil were fertilized with 320 kg of N ha−1 in form of sewage sludge. These losses occurred within a time span of 6 months (May to Oct) and were mainly attributed to the high N fertilizer input and not to the chemical composition of the fertilizers. As in our study, almost 90 % of the annual leaching from the W05/90 plot happened during the winter/spring 2010 there might be some site specific but until now unknown reason for a relatively high leaching flush. No direct N input by fertilization or any comparable input occurred and possible mineralization artifacts potentially produced by the installation of the suction lysimeters can also be excluded, since they were installed 4 months earlier.
Increased nitrate leaching may also favor N20 emissions . As part of a Diploma thesis, a series of five N20 measurement campaigns between June and Oct 2010 was conducted on all plots, except for W94/30 . Results indicate that N2O emissions increased after heavy rainfalls at the end of August and in September 2010 at the P09/90 plot. Here, maximum mean values in August reached emissions of 65.0 (±20.5 SD) μg N2O-N m−2 h−1, but values fell back to a baseline of below 20 μg N2O-N m−2 h−1 in October 2010 again. However, also the reference plot showed peak values during this period (August 2010: 43.5 ± 18.6 SD μg N2O-N m−2 h−1), whereas the W05/90 and P94/30 plots never had higher emissions than 20 μg N2O-N m−2 h−1 . In agreement with other studies [71, 72], it is concluded that SRC, once established, emit considerably less N2O, compared to other bioenergy crops or even less than fallow grounds . Estimated annual emission rates for the established SRC plots in the Fuhrberger Feld were below 1 kg ha−1 a−1 .
Former cropland which was abandoned due to protection reasons of nitrate pollution in lowland drinking water catchment areas can well be used for the cultivation of SRC to increase the land use value by the production of woody biomass. Our results showed that in SRCs of willow and poplar clones with different age (2–17 years) and different soil preparation measures (standard and deep plowing), the mean nitrate concentrations in 100 cm soil depth with few exceptions stays below the drinking water threshold value of 10.3 mg NO3-N L−1. There are two stages, where relatively increased amounts of nitrate might be leached from SRC cultivations, i.e., (1) when SRC are newly installed and intensive or even deep plowing was applied before cultivation (example P09/90) and (2) when the sink, respectively the export function for N compounds by tree uptake and harvesting measures is offset (example W94/30). Harvesting itself obviously did not initiate a nitrate flush, but nitrate release from an over-aged, never harvested willow stand was significantly increased.
Furthermore, we conclude that groundwater recharge rates, which are also of concern in drinking water sanctuaries, were not excessively reduced by SRC cultivations. Soils with low amounts of plant available soil water storage capacity and high permeability, as often found in lowland drinking water catchment areas, set a minimum level for groundwater recharge by limiting transpiration, as long as roots have no access to the groundwater table. Thus, less precipitation is needed to refill the soil water storage than on soils with higher water storage capacity, and groundwater recharge begins earlier after transpiration ceases in autumn.
Another finding, which needs more investigation though, might provide an opportunity to manipulate the water balance of SRC stands by management. Less interception loss can be expected from stands during the first year after harvested and thus higher groundwater recharge rates might be obtained by choosing a shorter rotation cycle, as the stand then is more often in the resprouting state.
The authors are grateful to the Hanover water authorities (Stadtwerke Hannover AG; www.enercity.de), to the Agency for Renewable Resources (FNR e.V;. www.fnr.de) and to the European ERA-NET-Bioenergy program (www.eranetbioenergy.net) for funding this study. We give our special thanks to D. Böttger for his field assistance, P.-E. Jansson for his valuable hints and suggestions concerning the water balance simulations and O. van Straaten for correcting grammar and spelling of the manuscript.
This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.