Comparison of Exfoliative Pap Stain and AgNOR Counts of the Tongue in Smokers and Nonsmokers
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Objective To compare exfoliative cytology from the oral mucosa of smokers and nonsmokers, with emphasis on proliferative activity. Methods Exfoliative cytology specimens were obtained from clinical normal mucosa from the lateral border of the tongue in 30 nonsmokers and 30 smokers ranging in age from 40 to 70 years of age, who were seen at the Heart Institute’s Patient Center and the Smoking Cessation Program of the University Hospital, University of São Paulo Medical School (InCor-HCFMUSP). The cytologic specimens were evaluated by Papanicolaou staining and AgNOR quantification in order to evaluate the presence of cytological alterations suggestive of inflammation, dysplasia, keratinization, and proliferative activity of epithelial cells. Results Only Papanicolaou Class I and Class II smears were observed. Inflammatory alterations were found in 90% of smokers and in 87% of nonsmokers. The number of AgNORs/nucleus differed significantly between smokers and nonsmokers (3.372 ± 0.375 versus 2.732 ± 0.236). Conclusions Within the limitations of this research, the results indicate higher proliferative activity in smoking patients compared to nonsmoking patients, even in the absence of clinical lesions.
- Banoczy J, Squier C. Smoking and disease. Eur J Dent Educ. 2004;8 Suppl 4:7–10. CrossRef
- Castellsague X, Quintana MJ, Martinez MC, Nieto A, Sanchez MJ, et al. The role of type of tobacco and type of alcoholic beverage in oral carcinogenesis. Int J Cancer. 2004;108(5):741–9. CrossRef
- Sapp JP, Eversole LR, Wysocki GP. Contemporary oral and maxillofacial pathology. Los Angeles: CV Mosby; 1997. p. 174–80.
- Taybos G. Oral changes associated with tobacco use. Am J Med Sci. 2003;326(4):179–82. CrossRef
- Pavanello MB, Prado FA, Balducci I, Brandão AAH, Almeida JD. Cytological analysis of alterations induced by smoking and by alcohol consumption. Acta Cytol. 2006;50(50):435–40.
- Winn DM. Tobacco use and oral diseases. J Dent Educ. 2001;65:306–12.
- Stewart BW, Kleihues P. World cancer report. International Agency for Research on Cancer. Lyon: IARC Press; 2003. p. 22–7, 172–4.
- BRASIL. Ministério da Saúde. Instituto Nacional do Câncer. Estimativas da incidência e da mortalidade por câncer no Brasil em 2006. Available at: http://www.inca.org.br/estimativas/2006/index.asp?=tabelaestados.asp
- Fontes PC, Kitakawa D, Carvalho YR, Brandão AA, Cabral LAG, Almeida JD. Sporotrichosis in an HIV-positive man with oral lesions: a case report. Acta Cytol. 2007;51:648–50.
- Almeida JD, Cabral LAG, Brandão AAG. Exfoliative cytology as a diagnostic method in Stomatology. J Dent Res. 1994;73:765.
- Cançado RP, Yurgel LS, Sant’anna F. Evaluation of the nucleolar organizer regions associated proteins in exfoliative cytology of normal buccal mucosa. Effect of smoking. Oral Oncol. 2001;37:446–54. CrossRef
- Ogden GR, Cowpe JG, Green MW. Quantitative exfoliative cytology of normal buccal mucosa: effect of smoking. J Oral Pathol. 1990;19:53–5. CrossRef
- Chattopadhyay A, Ray JG, Caplan DJ. AgNOR count as objective marker for dysplastic features in oral leukoplakia. J Oral Pathol Med. 2002;31(9):512–7. CrossRef
- Eslami B, Yaghmaei M, Firoozi M, Saffar AS. Nucleolar organizer regions in selected odontogenic lesions. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003;95(2):187–92. CrossRef
- Paiva RL, Sant′Ana Filho M, Bohrer PL, Lauxen Ida S, Rados PV. AgNOR quantification in cells of normal oral mucosa exposed to smoking and alcohol. A cytopathologic study. Anal Quant Cytol Histol. 2004;26(3):175–80.
- Remmerbach TW, Weidenbach H, Muller C, Hemprich A, Pomjanski N, et al. Diagnostic value of nucleolar organizer regions (AgNORs) in brush biopsies of suspicious lesions of the oral cavity. Anal Cell Pathol. 2003;25(3):139–46.
- Sethi P, Shah PM. Oral exfoliative cytology of smokers at discrete clinical stages using AgNOR staining. Indian J Dent Res. 2003;14(3):142–5.
- Howell WM, Denton TE, Diamond JR. Differential staining of the satellite regions of human acrocentric chromosomes. Experientia. 1975;31(2):260–2. CrossRef
- Gedoz L, Lauxen Ida S, Sant’Ana MF, Rados PV. Proliferative activity in clinically healthy oral mucosa exposed to tobacco smoking and alcohol: a longitudinal study using the AgNOR staining technique. Anal Quant Cytol Histol. 2007;29(4):231–8.
- Hoffmann D, Wynder EL. Chemical constituents and bioactivity of tobacco smoke. IARC Sci Publ. 1986;74:145–65.
- Trerè D. AgNOR staining and quantification. Micron. 2000;31:127–31. CrossRef
- Crocker J, Bold DAR, Egan MJ. How should we count AgNORs? Proposals for a standardized approach. J Pathol. 1989;158:185–8. CrossRef
- de Sampaio H C, Loyola AM, Gomez RS, Mesquita RA. AgNOR count in exfoliative cytology of normal buccal mucosa: effect of smoking. Acta Cytol. 1999;43(4):117–20.
- Orellana-Bustos AI, Espinoza-Santander IL, Franco-Martínez ME, Lobos-James-Freyre N, Ortega-Pinto AV. Evaluation of keratinization and AgNORs counts in exfoliative cytology of normal mucosa from smokers and non-smokers. Med Oral. 2004;9(5):197–203.
- Ploton D, Menager M, Jeannesson P, Himber G, Pigeon F, Adnet JJ. Improvements in the staining and in the visualization of the argyrophilic proteins of the nucleolar organizer region at the optical level. Histochem J. 1986;18:5–14. CrossRef
- Fontes PC, Côrrea GHM, Issa JS, Almeida JD. Quantitative analysis of AgNOR proteins in exfoliative cytology specimens of oral mucosa from smokers and non-smokers. Anal Quant Cytol Histol. 2008;30:16–24.
- Warnakulasuriya S, Reibel J, Bouquot J, Dabelsteen E. Oral epithelial dysplasia classification systems: predictive value, utility, weaknesses and scope for improvement. J Oral Pathol Med. 2008;37:127–33.
- Driemel O, Hertel K, Reichert T, Kosmehi H. Current classification of precursor lesions of oral squamous cell carcinoma principles of the WHO classification 2005. Mund Kiefer Gesichtschir. 2006;10(2):89–93. CrossRef
- Comparison of Exfoliative Pap Stain and AgNOR Counts of the Tongue in Smokers and Nonsmokers
Head and Neck Pathology
Volume 2, Issue 3 , pp 157-162
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- Nucleolar organizer region
- Disease prevention
- Oral mucosa
- Author Affiliations
- 1. Postgraduation Program in Oral Biopathology, São José dos Campos Dental School, UNESP – São Paulo State University, São José dos Campos, Sao Paulo, Brazil
- 2. São José dos Campos Dental School, UNESP – São Paulo State University, São José dos Campos, Sao Paulo, Brazil
- 3. Smoking Cessation Program, Heart Institute (InCor), Medical School, University of São Paulo, Sao Paulo, SP, Brazil
- 4. Department of Biosciences and Oral Diagnosis, São José dos Campos Dental School, UNESP – São Paulo State University, São José dos Campos, Av. Francisco José Longo, 777 São Dimas, Sao Paulo, 12245-000, Brazil