Original Paper

Head and Neck Pathology

, 2:157

First online:

Comparison of Exfoliative Pap Stain and AgNOR Counts of the Tongue in Smokers and Nonsmokers

  • Patrícia Campos FontesAffiliated withPostgraduation Program in Oral Biopathology, São José dos Campos Dental School, UNESP – São Paulo State University
  • , Gustavo Henrique Marques CorrêaAffiliated withSão José dos Campos Dental School, UNESP – São Paulo State University
  • , Jaqueline Scholz IssaAffiliated withSmoking Cessation Program, Heart Institute (InCor), Medical School, University of São Paulo
  • , Adriana Aigotti Haberbeck BrandãoAffiliated withDepartment of Biosciences and Oral Diagnosis, São José dos Campos Dental School, UNESP – São Paulo State University
  • , Janete Dias AlmeidaAffiliated withDepartment of Biosciences and Oral Diagnosis, São José dos Campos Dental School, UNESP – São Paulo State University Email author 

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Abstract

Objective To compare exfoliative cytology from the oral mucosa of smokers and nonsmokers, with emphasis on proliferative activity. Methods Exfoliative cytology specimens were obtained from clinical normal mucosa from the lateral border of the tongue in 30 nonsmokers and 30 smokers ranging in age from 40 to 70 years of age, who were seen at the Heart Institute’s Patient Center and the Smoking Cessation Program of the University Hospital, University of São Paulo Medical School (InCor-HCFMUSP). The cytologic specimens were evaluated by Papanicolaou staining and AgNOR quantification in order to evaluate the presence of cytological alterations suggestive of inflammation, dysplasia, keratinization, and proliferative activity of epithelial cells. Results Only Papanicolaou Class I and Class II smears were observed. Inflammatory alterations were found in 90% of smokers and in 87% of nonsmokers. The number of AgNORs/nucleus differed significantly between smokers and nonsmokers (3.372 ± 0.375 versus 2.732 ± 0.236). Conclusions Within the limitations of this research, the results indicate higher proliferative activity in smoking patients compared to nonsmoking patients, even in the absence of clinical lesions.

Keywords

Cytodiagnosis Smoking Nucleolar organizer region Disease prevention Oral mucosa