Abstract
The apo-form of the 23.3 kDa catalytic domain of the AA9 family lytic polysaccharide monooxygenase NcLPMO9C from Neurospora crassa has been isotopically labeled and recombinantly expressed in Pichia pastoris. In this paper, we report the 1H, 13C, and 15N chemical shift assignments of this LPMO.
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Acknowledgments
This work was financed by SO-funds from the Norwegian University of Science and Technology (NTNU) and by the MARPOL project, the Norwegian NMR Platform and a FRINAT Project, all from the Research Council of Norway (Grant Numbers 221576, 226244, and 214613, respectively). The NMR laboratory at Aalborg University is supported by the Obel, SparNord and Carlsberg Foundations.
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Courtade, G., Wimmer, R., Dimarogona, M. et al. Backbone and side-chain 1H, 13C, and 15N chemical shift assignments for the apo-form of the lytic polysaccharide monooxygenase NcLPMO9C. Biomol NMR Assign 10, 277–280 (2016). https://doi.org/10.1007/s12104-016-9683-x
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DOI: https://doi.org/10.1007/s12104-016-9683-x