Biomolecular NMR Assignments

, Volume 8, Issue 1, pp 201–205

1H, 13C, 15N backbone and side chain NMR resonance assignments of the N-terminal NEAr iron transporter domain 1 (NEAT 1) of the hemoglobin receptor IsdB of Staphylococcus aureus

  • Brittany A. Fonner
  • Brian P. Tripet
  • Mengyao Lui
  • Hui Zhu
  • Benfang Lei
  • Valérie Copié
Article

DOI: 10.1007/s12104-013-9483-5

Cite this article as:
Fonner, B.A., Tripet, B.P., Lui, M. et al. Biomol NMR Assign (2014) 8: 201. doi:10.1007/s12104-013-9483-5

Abstract

Staphylococcus aureus is an opportunistic pathogen that causes skin and severe infections in mammals. Critical to S. aureus growth is its ability to scavenge iron from host cells. To this effect, S. aureus has evolved a sophisticated pathway to acquire heme from hemoglobin (Hb) as a preferred iron source. The pathway is comprised of nine iron-regulated surface determinant (Isd) proteins involved in heme capture, transport, and degradation. A key protein of the heme acquisition pathway is the surface-anchored hemoglobin receptor protein IsdB, which is comprised of two NEAr transporter (NEAT) domains that act in concert to bind Hb and extract heme for subsequent transfer to downstream acquisition pathway proteins. Despite significant advances in the structural knowledge of other Isd proteins, the structural mechanisms and molecular basis of the IsdB-mediated heme acquisition process are not well understood. In order to provide more insights into the mode of function of IsdB, we have initiated NMR structural studies of the first NEAT domain of IsdB (IsdBN1). Herein, we report the near complete 1H, 13C and 15N resonance assignments of backbone and side chain atoms, and the secondary structural topology of the 148-residue IsdB NEAT 1 domain. The NMR results are consistent with the presence of eight β-strands and one α-helix characteristic of an immunoglobulin-like fold observed in other NEAT domain family proteins. This work provides a solid framework to obtain atomic-level insights toward understanding how IsdB mediates IsdB-Hb protein–protein interactions critical for heme capture and transfer.

Keywords

Staphylococcus aureus NEAr transporter (NEAT) domains IsdB protein NMR resonance assignments Protein secondary structure Bacterial heme acquisition pathway Iron surface determinant (Isd) proteins 

Abbreviations

PMSF

Phenylmethylsulfonyl fluoride

EDTA

Ethylenediamenetetraacetic acid

DSS

4,4-dimethyl-4-silopentane-1-sulfonic acid

IPTG

Isopropyl-thio-β-galactoside

IsdB

Iron surface determinant protein B

IsdBN1

N-terminal NEAr iron transporter domain 1 (NEAT 1) of IsdB

IsdHN1

NEAT 1 domain of IsdH

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Brittany A. Fonner
    • 1
  • Brian P. Tripet
    • 1
  • Mengyao Lui
    • 2
  • Hui Zhu
    • 2
  • Benfang Lei
    • 2
  • Valérie Copié
    • 1
  1. 1.Department of Chemistry and BiochemistryMontana State UniversityBozemanUSA
  2. 2.Department of Immunology and Infectious DiseasesMontana State UniversityBozemanUSA

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