Biomolecular NMR Assignments

, Volume 7, Issue 1, pp 85–88

1H, 13C and 15N resonance assignments of the C-terminal DNA-binding domain of RstA protein from Klebsiella pneumoniae

  • Sheng-Chia Chen
  • Chi-Fon Chang
  • Pei-Ju Fan
  • Ya-Hsin Cheng
  • Tsunai Yu
  • Tai-huang Huang
Article

DOI: 10.1007/s12104-012-9383-0

Cite this article as:
Chen, SC., Chang, CF., Fan, PJ. et al. Biomol NMR Assign (2013) 7: 85. doi:10.1007/s12104-012-9383-0

Abstract

Bacterial cells often use two-component signal transduction systems to regulate genes in response to environmental stimuli. The RstA/RstB system is a two-component regulatory system consisting of the membrane sensor, RstB, and its cognate response regulator RstA. The RstA of Klebsiella pneumoniae consists of a N-terminal receiver domain (NRD, residues 1-119) and a C-terminal DNA-binding domain (DBD, residues 130-236). Phosphorylation of the response regulator induces a conformational change in the regulatory domain of RstA, which results in activation of the effector domain to regulate the downstream genes, including the ferrous iron transport system (Feo), at low-pH condition. Here we report the 1H, 13C and 15N resonance assignments and secondary structure identification of the DBD of RstA from K. pneumoniae as a first step for unraveling the structural and functional relationship of the RstA/RstB two component system.

Keywords

Two component systems DNA-binding Feo Ferrous iron transport Klebsiella pneumoniae 

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Sheng-Chia Chen
    • 1
  • Chi-Fon Chang
    • 2
  • Pei-Ju Fan
    • 1
  • Ya-Hsin Cheng
    • 1
  • Tsunai Yu
    • 1
  • Tai-huang Huang
    • 1
    • 2
    • 1
    • 2
    • 3
  1. 1.Institute of Biomedical SciencesAcademia SinicaTaipeiTaiwan, ROC
  2. 2.Genomics Research CenterAcademia SinicaTaipeiTaiwan, ROC
  3. 3.Department of PhysicsNational Taiwan Normal UniversityTaipeiTaiwan, ROC