Biomolecular NMR Assignments

, Volume 5, Issue 2, pp 169–175

Backbone assignment and secondary structure of the PsbQ protein from Photosystem II

  • Michaela Horničáková
  • Jaroslava Kohoutová
  • Judith Schlagnitweit
  • Christian Wohlschlager
  • Rüdiger Ettrich
  • Radovan Fiala
  • Wolfgang Schoefberger
  • Norbert Müller
Article

DOI: 10.1007/s12104-011-9293-6

Cite this article as:
Horničáková, M., Kohoutová, J., Schlagnitweit, J. et al. Biomol NMR Assign (2011) 5: 169. doi:10.1007/s12104-011-9293-6
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Abstract

PsbQ is one of the extrinsic proteins situated on the lumenal surface of photosystem II (PSII) in the higher plants and green algae. Its three-dimensional structure was determined by X-ray crystallography with exception of the residues 14–33. To obtain further details about its structure and potentially its dynamics, we approached the problem by NMR. In this paper we report 1H, 15N, and 13C NMR assignments for the PsbQ protein. The very challenging oligo-proline stretches could be assigned using 13C-detected NMR experiments that enabled the assignments of twelve out of the thirteen proline residues of PsbQ. The identification of PsbQ secondary structure elements on the basis of our NMR data was accomplished with the programs TALOS+, web server CS23D and CS-Rosetta. To obtain additional secondary structure information, three-bond HN-Hα J-coupling constants and deviation of experimental 13Cα and 13Cβ chemical shifts from random coil values were determined. The resulting “consensus” secondary structure of PsbQ compares very well with the resolved regions of the published X-ray crystallographic structure and gives a first estimate of the structure of the “missing link” (i.e. residues 14–33), which will serve as the basis for the further investigation of the structure, dynamics and interactions.

Keywords

Photosystem IIPsbQMissing linkNMR resonance assignmentProtein–protein interaction

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Michaela Horničáková
    • 1
  • Jaroslava Kohoutová
    • 2
    • 7
  • Judith Schlagnitweit
    • 1
  • Christian Wohlschlager
    • 3
  • Rüdiger Ettrich
    • 2
    • 4
  • Radovan Fiala
    • 5
  • Wolfgang Schoefberger
    • 6
  • Norbert Müller
    • 1
  1. 1.Institute of Organic ChemistryJohannes Kepler UniversityLinzAustria
  2. 2.Institute of Systems Biology and EcologyAcademy of Sciences of the Czech RepublicNové HradyCzech Republic
  3. 3.Virtual Reality ServicesJohannes Kepler UniversityLinzAustria
  4. 4.Faculty of SciencesUniversity of South BohemiaNové HradyCzech Republic
  5. 5.National Centre for Biomolecular ResearchMasaryk UniversityBrnoCzech Republic
  6. 6.Institute of Inorganic Chemistry, Center of Nanobionics and Photochemical Sciences (CNPS)LinzAustria
  7. 7.Institute of Physical BiologyUniversity of South BohemiaNové HradyCzech Republic