Biomolecular NMR Assignments

, Volume 3, Issue 2, pp 227-230

First online:

1H, 13C and 15N resonance assignments of a ribonucleoprotein complex consisting of Prp24-RRM2 bound to a fragment of U6 RNA

  • Stephen Martin-TumaszAffiliated withDepartment of Biochemistry, University of Wisconsin-Madison
  • , Samuel E. ButcherAffiliated withDepartment of Biochemistry, University of Wisconsin-Madison Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access


Saccharomyces cerevisiae Prp24 is an essential RNA binding protein involved in pre-mRNA splicing. Nearly complete backbone and side chain resonance assignments have been obtained for the second RNA recognition motif (RRM) of Prp24 (RRM2, residues M114-E197) both in isolation and bound to a six nucleotide fragment of U6 RNA (AGAGAU). In addition, nearly complete backbone assignments have been made for a Prp24 construct spanning the second and third RRMs (RRM23, residues M114-K290), both free and bound to AGAGAU.


RRM Protein–RNA complex Splicing snRNP