Biomolecular NMR Assignments

, 3:227

1H, 13C and 15N resonance assignments of a ribonucleoprotein complex consisting of Prp24-RRM2 bound to a fragment of U6 RNA


DOI: 10.1007/s12104-009-9181-5

Cite this article as:
Martin-Tumasz, S. & Butcher, S.E. Biomol NMR Assign (2009) 3: 227. doi:10.1007/s12104-009-9181-5


Saccharomyces cerevisiae Prp24 is an essential RNA binding protein involved in pre-mRNA splicing. Nearly complete backbone and side chain resonance assignments have been obtained for the second RNA recognition motif (RRM) of Prp24 (RRM2, residues M114-E197) both in isolation and bound to a six nucleotide fragment of U6 RNA (AGAGAU). In addition, nearly complete backbone assignments have been made for a Prp24 construct spanning the second and third RRMs (RRM23, residues M114-K290), both free and bound to AGAGAU.


RRMProtein–RNA complexSplicingsnRNP

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© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Department of BiochemistryUniversity of Wisconsin-MadisonMadisonUSA