Clinical and Translational Oncology

, Volume 14, Issue 3, pp 232–236

Tissue microarray-based study of patients with lymph node-positive breast cancer shows tyrosine phosphorylation of signal transducer and activator of transcription 3 (tyrosine705-STAT3) is a marker of good prognosis

  • Amir Sonnenblick
  • Anat Shriki
  • Eithan Galun
  • Jonathan H. Axelrod
  • Hagit Daum
  • Yakir Rottenberg
  • Tamar Hamburger
  • Bela Mali
  • Tamar Peretz
Research Articles

DOI: 10.1007/s12094-012-0789-z

Cite this article as:
Sonnenblick, A., Shriki, A., Galun, E. et al. Clin Transl Oncol (2012) 14: 232. doi:10.1007/s12094-012-0789-z

Abstract

Background

Although lymph node-positive breast cancers are associated with poorer prognosis, individual patients may have different clinical outcomes. Signal transducer and activator of transcription 3 (STAT3) is a point of convergence for numerous oncogenic signalling pathways. The goal of this study was to determine the prognostic value of phosphorylated (tyrosine705)-STAT3 in node-positive breast cancer patients.

Methods

Immunohistochemical analysis of Phospho-STAT3 was performed on a tissue microarray of breast cancer specimens. The expression pattern of Phospho-STAT3 was correlated with survival outcome, and clinical and pathological parameters.

Results

Out of 125 interpretable tumours, positive Phospho-STAT3 nuclear expression was seen in 35 (28%) of tumours. There was no significant relationship between Phospho-STAT3 expression and clinical-pathological parameters including age, hormonal receptor status, grade and tumour size. Interestingly positive tumours had a significantly improved disease-free survival at 5 years (p=0.035). Additionally, positive Phospho-STAT3 nuclear expression was correlated with significantly improved survival at both 5 years (p=0.023) and 10 years (p=0.026). Finally, in multivariate analyses Phospho-STAT3 was found to be an independent prognostic marker of overall survival in node-positive breast cancer patients.

Conclusion

These findings support the role of Phospho-STAT3 as an important independent prognostic marker in node-positive breast cancer patients.

Keywords

Breast cancerPhosphorylationSTAT3Tissue microarrayImmunohistochemistry

Copyright information

© Feseo 2012

Authors and Affiliations

  • Amir Sonnenblick
    • 1
    • 2
  • Anat Shriki
    • 2
  • Eithan Galun
    • 2
  • Jonathan H. Axelrod
    • 2
  • Hagit Daum
    • 3
  • Yakir Rottenberg
    • 1
  • Tamar Hamburger
    • 1
  • Bela Mali
    • 4
  • Tamar Peretz
    • 1
  1. 1.Sharett Institute of OncologyHadassah-Hebrew University Medical CenterJerusalemIsrael
  2. 2.Goldyne Savad Institute of Gene TherapyHadassah-Hebrew University Medical CenterJerusalemIsrael
  3. 3.Department of Obstetrics and GynecologyHadassah-Hebrew University Medical CenterMt Scopus, JerusalemIsrael
  4. 4.Department of PathologyHadassah-Hebrew University HospitalJerusalemIsrael