Indian Journal of Microbiology

, Volume 52, Issue 3, pp 316–324

Mass Production of the Beneficial Nematode Heterorhabditis bacteriophora and Its Bacterial Symbiont Photorhabdus luminescens

  • Floyd L. InmanIII
  • Sunita Singh
  • Leonard D. Holmes
Review Article

DOI: 10.1007/s12088-012-0270-2

Cite this article as:
Inman, F.L., Singh, S. & Holmes, L.D. Indian J Microbiol (2012) 52: 316. doi:10.1007/s12088-012-0270-2

Abstract

Entomoparasitic nematodes (EPNs) are being commercialized as a biocontrol measure for crop insect pests, as they provide advantages over common chemical insecticides. Mass production of these nematodes in liquid media has become a major challenge for commercialization. Producers are not willing to share the trade secrets of mass production and by doing so, have made culturing EPNs extremely difficult to advance existing technologies. Theoretically, mass production in liquid media is an ideal culturing method as it increases cost efficiency and nematode quantity. This paper will review current culturing methodologies and suggest basic culturing parameters for mass production. This review is focused on Heterorhabditis bacteriophora; however, this information can be useful for other nematode species.

Keywords

Beneficial nematodesPhotorhabdus luminescensHeterorhabditis bacteriophoraMass production

Copyright information

© Association of Microbiologists of India 2012

Authors and Affiliations

  • Floyd L. InmanIII
    • 1
  • Sunita Singh
    • 2
  • Leonard D. Holmes
    • 1
  1. 1.Sartorius-Stedim Biotechnology Laboratory, Biotechnology Research and Training CenterUniversity of North Carolina at PembrokePembrokeUSA
  2. 2.Central Institute of Agricultural EngineeringBhopalIndia