Indian Journal of Microbiology

, Volume 52, Issue 3, pp 366–372

Antimicrobial Agents Produced by Marine Aspergillus terreus var. africanus Against Some Virulent Fish Pathogens

Original Article

DOI: 10.1007/s12088-012-0255-1

Cite this article as:
Barakat, K.M. & Gohar, Y.M. Indian J Microbiol (2012) 52: 366. doi:10.1007/s12088-012-0255-1


Screening of fungal isolates collected from different locations of Alexandria coast, Egypt, was carried out to obtain new biologically active metabolites against some virulent fish pathogens (Edwardsiella tarda, Aeromonas hydrophila, Vibrio ordalli and Vibrio angularuim). Among 26 fungal isolates, Aspergillus terreus var. africanus was identified as the most potent isolate. Production of the bioactive material was optimized using response surface methodology including fermentation media, incubation period, temperature, pH, and thermo-stability. Spectral properties of the gas chromatography/mass spectrum of the ethyl acetate crude extract were determined. Partially purified components of the crude extract were chromatographically separated and bioassayed. Out of ten separated compounds, five were with considerable antibacterial agent. The bio-toxicity of crude showed a slight toxicity against the brine shrimp Artemia salina (LC50 = 1,500 μg/l). Antibacterial activity of the crude was compared with some known standard antibiotics and found to be superior over many where its MIC against some pathogen reached 1 μg/ml.


Marine fungiAntimicrobial agentsFish pathogensResponse surfaceGC/MSBio-toxicity

Copyright information

© Association of Microbiologists of India 2012

Authors and Affiliations

  1. 1.Microbiology LabNational Institute of Oceanography and FisheriesAlexandriaEgypt
  2. 2.Botany and Microbiology Department, Faculty of ScienceAlexandria UnivAlexandriaEgypt