Indian Journal of Microbiology

, Volume 51, Issue 1, pp 54–60

Antifungal Activity in Ethanolic Extracts of Carica papaya L. cv. Maradol Leaves and Seeds

  • Pedro Chávez-Quintal
  • Tania González-Flores
  • Ingrid Rodríguez-Buenfil
  • Santiago Gallegos-Tintoré
Original Article

DOI: 10.1007/s12088-011-0086-5

Cite this article as:
Chávez-Quintal, P., González-Flores, T., Rodríguez-Buenfil, I. et al. Indian J Microbiol (2011) 51: 54. doi:10.1007/s12088-011-0086-5

Abstract

Bioactive compounds from vegetal sources are a potential source of natural antifungic. An ethanol extraction was used to obtain bioactive compounds from Carica papaya L. cv. Maradol leaves and seeds of discarded ripe and unripe fruit. Both, extraction time and the papaya tissue flour:organic solvent ratio significantly affected yield, with the longest time and highest flour:solvent ratio producing the highest yield. The effect of time on extraction efficiency was confirmed by qualitative identification of the compounds present in the lowest and highest yield extracts. Analysis of the leaf extract with phytochemical tests showed the presence of alkaloids, flavonoids and terpenes. Antifungal effectiveness was determined by challenging the extracts (LE, SRE, SUE) from the best extraction treatment against three phytopathogenic fungi: Rhizopus stolonifer, Fusarium spp. and Colletotrichum gloeosporioides. The leaf extract exhibited the broadest action spectrum. The MIC50 for the leaf extract was 0.625 mg ml−1 for Fusarium spp. and >10 mg ml−1 for C. gloeosporioides, both equal to approximately 20% mycelial growth inhibition. Ethanolic extracts from Carica papaya L. cv. Maradol leaves are a potential source of secondary metabolites with antifungal properties.

Keywords

Papaya harvest by-productsAntifungal compoundsPhytopathogen inhibitionEthanolic extraction

Copyright information

© Association of Microbiologists of India 2011

Authors and Affiliations

  • Pedro Chávez-Quintal
    • 1
  • Tania González-Flores
    • 1
  • Ingrid Rodríguez-Buenfil
    • 1
  • Santiago Gallegos-Tintoré
    • 1
  1. 1.Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C.Unidad SuresteMéridaMéxico