Original Article

Indian Journal of Microbiology

, Volume 47, Issue 1, pp 26-29

First online:

Paddy straw as substrate for ethanol production

  • L. WatiAffiliated withDepartment of Microbiology, CCS Haryana Agricultural University Email author 
  • , S. KumariAffiliated withDepartment of Microbiology, CCS Haryana Agricultural University
  • , B. S. KunduAffiliated withDepartment of Microbiology, CCS Haryana Agricultural University

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Abstract

Pretreatment of paddy straw with 2% sodium hydroxide at 15 psi for 1 h resulted in 83% delignification. The hydrolysis of alkali treated paddy straw with a commercial preparation of cellulase for 2 h at 50°C resulted in release of 65% total reducing sugars. Maximum sugars were released at enzyme loading of 1.5% (v/v). The fermentation of hydrolysate supplemented with nutrients by S. cerevisiae resulted in the production of 20–30 g L−1 ethanol after 48 h incubation which was further improved with addition of yeast nitrogen base and inoculated with 1% (w/v) yeast cells.

Key words

Cellulose ethanol fermentation paddy straw S. cerevisiae