Hepatology International

, Volume 7, Issue 2, pp 516–523

Oxidative stress is closely associated with insulin resistance in genotypes 1 and 3 chronic hepatitis C

  • Said M. Hashemi
  • David van der Poorten
  • Francisco Barrera
  • Priyanka Bandara
  • Ora Lux
  • James Kench
  • Jacob George
Original Article

DOI: 10.1007/s12072-012-9400-5

Cite this article as:
Hashemi, S.M., van der Poorten, D., Barrera, F. et al. Hepatol Int (2013) 7: 516. doi:10.1007/s12072-012-9400-5

Abstract

Background

Chronic hepatitis C (CHC) infection is associated with insulin resistance and with oxidative stress, but the relationship between the two has not been thoroughly examined.

Purpose

To evaluate the association between insulin resistance and oxidative stress in CHC patients.

Method

In 115 CHC patients (68 with genotype 1 and 47 with genotype 3), the relationship between the serum concentration of malondialdehyde (MDA), a marker of oxidative stress and insulin resistance as defined by the homeostasis model (HOMA-IR) was examined.

Results

There was no significant difference in MDA levels between genotype 1- and genotype 3-infected subjects (12.882 vs. 12.426 ng/mL, p = 0.2). By univariate analysis, factors associated with HOMA-IR in both genotypes were oxidative stress as measured by MDA (p = 0.002), body mass index (BMI), portal activity, and fibrosis. Genotype-specific differences in HOMA-IR association were steatosis and triglycerides (TG) for genotype 1, and age and glutathione (GSH) for genotype 3. In a stepwise multiple linear regression analysis in both genotypes, MDA was a significant and independent predictor of HOMA-IR (p = 0.04). As expected, BMI and fibrosis were likewise independently correlated to HOMA-IR. In addition, MDA levels were higher (p < 0.001) and GSH levels were lower (p = 0.023) in insulin-resistant subjects compared to their insulin-sensitive counterparts.

Conclusions

It is concluded that in CHC, oxidative stress is an independent predictor of HOMA-IR, irrespective of virus genotype. Further studies on the role of oxidative stress in the development of insulin resistance in CHC are warranted.

Keywords

Hepatitis CInsulin resistanceMalondialdehydeOxidative stress

Abbreviations

CHC

Chronic hepatitis C

MDA

Malondialdehyde

GSH

Glutathione

HOMA-IR

Homeostasis model assessment of insulin resistance

BMI

Body mass index

WHR

Waist/hip ratio

IR

Insulin resistance

HCC

Hepatocellular carcinoma

HCV

Hepatitis C virus

IR

Insulin resistance

ROS

Reactive oxygen species

ALT

Alanine aminotransferase

AST

Aspartate aminotransferase

GGT

Gamma-glutamyl transpeptidase

T-chol

Total cholesterol

HDL-C

High density lipoprotein cholesterol

LDL-C

Low density lipoprotein cholesterol

TG

Triglycerides

HIV

Human immunodeficiency virus

HPLC

High performance liquid chromatography

SD

Standard deviation

DNPH

Dinitrophenylhydrazine

UV

Ultraviolet

NS3

Non-structural protein 3

NS5A

Non-structural protein 5A

SOCS-3

Suppressor of cytokine signaling-3

NF-κβ

Nuclear factor-κβ

TNF-α

Tumor necrosis factor-α

Copyright information

© Asian Pacific Association for the Study of the Liver 2012

Authors and Affiliations

  • Said M. Hashemi
    • 1
  • David van der Poorten
    • 1
  • Francisco Barrera
    • 1
    • 2
  • Priyanka Bandara
    • 1
  • Ora Lux
    • 3
  • James Kench
    • 4
  • Jacob George
    • 1
  1. 1.Storr Liver Unit, Westmead Millennium InstituteUniversity of Sydney at Westmead HospitalSydneyAustralia
  2. 2.Departamento de GastroenterologíaPontificia Universidad CatólicaSantiagoChile
  3. 3.Department of Clinical ChemistryPrince of Wales HospitalSydneyAustralia
  4. 4.Department of Anatomical PathologyRoyal Prince Alfred HospitalSydneyAustralia