Tropical Plant Biology

, Volume 1, Issue 3, pp 278–292

Genome-Wide Comparative Analyses of Microsatellites in Papaya

  • Jianping Wang
  • Cuixia Chen
  • Jong-Kuk Na
  • Qingyi Yu
  • Shaobin Hou
  • Robert E. Paull
  • Paul H. Moore
  • Maqsudul Alam
  • Ray Ming
Article

DOI: 10.1007/s12042-008-9024-z

Cite this article as:
Wang, J., Chen, C., Na, JK. et al. Tropical Plant Biol. (2008) 1: 278. doi:10.1007/s12042-008-9024-z

Abstract

Microsatellites, or simple sequence repeats (SSRs), are highly polymorphic and universally distributed in eukaryotes. SSRs have been used extensively as sequence tagged markers in genetic studies. Recently, the functional and evolutionary importance of SSRs has received considerable attention. Here we report the mining and characterization of the SSRs in papaya genome. We analyzed SSRs from 277.4 Mb of whole genome shotgun (WGS) sequences, 51.2 Mb bacterial artificial chromosome (BAC) end sequences (BES), and 13.4 Mb expressed sequence tag (EST) sequences. The papaya SSR density was one SSR per 0.7 kb of DNA sequence in the WGS, which was higher than that in BES and EST sequences. SSR abundance was dramatically reduced as the repeat length increased. According to SSR motif length, dinucleotide repeats were the most common motif in class I, whereas hexanucleotides were the most copious in class II SSRs. The tri- and hexanucleotide repeats of both classes were greater in EST sequences compared to genomic sequences. In class I SSR, AT and AAT were the most frequent motifs in BES and WGS sequences. By contrast, AG and AAG were the most abundant in EST sequences. For SSR marker development, 9,860 primer pairs were surveyed for amplification and polymorphism. Successful amplification and polymorphic rates were 66.6% and 17.6%, respectively. The highest polymorphic rates were achieved by AT, AG, and ATG motifs. The genome wide analysis of microsatellites revealed their frequency and distribution in papaya genome, which varies among plant genomes. This complete set of SSRs markers throughout the genome will assist diverse genetic studies in papaya and related species.

Keywords

Bacterial artificial chromosome end sequences (BES) Carica papaya Expressed sequence tag (EST) Motif Simple sequence repeats (SSRs) Whole genome shotgun (WGS) sequences 

Supplementary material

12042_2008_9024_MOESM1_ESM.xls (3 mb)
Supplementary Table 1Comprehensive information of 9,860 SSR marker surveyed (DOC 3.04 MB)

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Jianping Wang
    • 1
  • Cuixia Chen
    • 1
  • Jong-Kuk Na
    • 1
  • Qingyi Yu
    • 2
  • Shaobin Hou
    • 3
  • Robert E. Paull
    • 4
  • Paul H. Moore
    • 5
  • Maqsudul Alam
    • 3
  • Ray Ming
    • 1
  1. 1.Department of Plant BiologyUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  2. 2.Hawaii Agriculture Research CenterAieaUSA
  3. 3.Advanced Studies in Genomics, Proteomics and BioinformaticsUniversity of HawaiiHonoluluUSA
  4. 4.Department of Tropical Plant and Soil SciencesUniversity of HawaiiHonoluluUSA
  5. 5.USDA-ARSPacific Basin Agricultural Research CenterHiloUSA

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