Journal of Biosciences

, Volume 38, Issue 3, pp 523–532

MiR-200a enhances the migrations of A549 and SK-MES-1 cells by regulating the expression of TSPAN1

Article

DOI: 10.1007/s12038-013-9351-6

Cite this article as:
Chen, Y., Peng, W., Lu, Y. et al. J Biosci (2013) 38: 523. doi:10.1007/s12038-013-9351-6

Abstract

MicroRNA-200a (miR-200a) has been reported to regulate tumour progression in several tumours; however, little is known about its role in non-small cell lung cancer cells (NSCLCs). Here, we found that miR-200a was up-regulated in A549 and SK-MES-1 cells compared with normal lung cells HELF. By a series of gain-of-function and loss-of-function studies, over-expression of miR-200a was indicated to enhance cells migration, and its knock-down inhibited migration of cells in NSCLC cell lines. Furthermore, miR-200a was identified to induce TSPAN1 expression which was related to migration. TSPAN1 was proved to induce migration, and so up-regulation of TSPAN1 by miR-200a may explain why over-expressing miR-200a promotes NSCLC cells migration.

Keywords

MicroRNA migration miR-200a NSCLC TSPAN1 

Copyright information

© Indian Academy of Sciences 2013

Authors and Affiliations

  1. 1.School of Life Science and TechnologyChina Pharmaceutical UniversityNanjingChina
  2. 2.Biomics Biotechnologies Co. LtdNantongChina