Journal of Biosciences

, Volume 36, Issue 2, pp 289–296

Genome-wide screening reveals the emergence and divergence of RTK homologues in basal Metazoan Hydra magnipapillata

Article

DOI: 10.1007/s12038-011-9065-6

Cite this article as:
Reddy, P., Bidaye, S.S. & Ghaskadbi, S. J Biosci (2011) 36: 289. doi:10.1007/s12038-011-9065-6

Abstract

Receptor tyrosine kinases (RTKs) are key components of cell–cell signalling required for growth and development of multicellular organisms. It is therefore likely that the divergence of RTKs and associated components played a significant role in the evolution of multicellular organisms. We have carried out the present study in hydra, a diploblast, to investigate the divergence of RTKs after parazoa and before emergence of triploblast phyla. The domain-based screening using Hidden Markov Models (HMMs) for RTKs in Genomescan predicted gene models of the Hydra magnipapillata genome resulted in identification of 15 RTKs. These RTKs have been classified into eight families based on domain architecture and homology. Only 5 of these RTKs have been previously reported and a few of these have been partially characterized. A phylogeny-based analysis of these predicted RTKs revealed that seven subtype duplications occurred between ‘parazoan–eumetazoan split’ and ‘diploblast–triploblast split’ in animal phyla. These results suggest that most of the RTKs evolved before the radiata–bilateria divergence during animal evolution.

Keywords

Evolution hydra phylogeny RTKs 

Abbreviations used

CA

Celera Assembler

ECD

extracellular domain

DDR

discoidin domain receptor tyrosine kinase

FGFR

fibroblast growth factor receptor

HMMs

Hidden Markov Models

JTT

Jones–Taylor–Thornton

ME

minimum evolution

MuSK

muscle-specific kinase

PCR

polymerase chain reaction

Ror

receptor tyrosine kinase–like orphan receptor

RP

Ringer-Phrap

RTKs

receptor tyrosine kinases

RyK

Wnt inhibitory receptor tyrosine kinase

TK

tyrosine kinase

TM

transmembrane

Supplementary material

12038_2011_9065_MOESM1_ESM.pdf (1 mb)
ESM 1(PDF 1.03 mb)

Copyright information

© Indian Academy of Sciences 2011

Authors and Affiliations

  • PC Reddy
    • 1
  • Salil S Bidaye
    • 1
    • 2
  • Surendra Ghaskadbi
    • 1
  1. 1.Division of Animal SciencesAgharkar Research InstitutePuneIndia
  2. 2.Research Institute of Molecular PathologyViennaAustria

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