Abstract
With increasing global competitions there is a growing need to develop environmentally benevolent nanoparticles without the use of toxic chemicals. The biosynthesis of silver nanoparticles (AgNPs) using plant extracts became one of the potential areas of research. The bioreduction of metal ion is quite rapid, readily perform at room temperature and easily scale up. The present study describes a rapid and eco-friendly synthesis of AgNPs using Eclipta alba plant extract in a single pot process. The efficiency and the influence of various process variables in the biosynthesis of AgNPs analysed include redundant concentration, temperature and time. AgNPs were rapidly synthesized using aqueous leaf extract of E. alba and was observed when the medium turned to brown colour with the addition of silver ion. Biosynthesized AgNPs were characterized by the help of UV–visible spectroscopy for their stability and physicochemical parameters were studied by dynamic light scattering, Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy. The obtained results confirmed that recorded UV spectra show the characteristic surface plasmon resonance band for AgNPs in the range of 400–440 nm and physiochemical structural analysis shown that obtained AgNPs were crystalline in nature. Further, cytotoxic and antimicrobial activities of biosynthesized AgNPs against RAW 254.7, MCF-7 and Caco-2 cells as well as Gram positive and Gram negative bacteria were assessed. In-vitro cytotoxicity activity of characterized AgNPs against tested cell lines showed significant anti-cell-proliferation effect in nanomolar concentrations. The antibacterial activity of synthesized AgNPs showed effective inhibitory activity against human pathogens, including, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. Thus, the significant outcome of this study would help to formulate value added herbal-based nano-materials in biomedical and nanotechnology industries.
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References
Robert T, Morrison Robert N, Boyd and Robert K 1992 Organic chemistry, 6th edition
Catauro M, Raucci M G, de Gaetano F and Marotta A 2004 J. Mater. Sci: Mater. Med. 15 831
Crabtree J H, Burchette R J, Siddiqi R A, Huen I T, Handott L L and Fishman A 2003 Peritoneal Dialysis Int. 23 368
Krolikowska A, Kudelski A, Michota A and Bukowska J 2003 Surf. Sci. Rep. 532 227
Zhao G J and Stevens S E 1998 Biometals 11 27
Daniel M C and Astruc D 2004 Chem. Rev. 104 293
Kato H 2011 Nat. Nanotechnol. 6 139
Mittal A K, Chisti Y and Banerjee U C 2013 Biotechnol. Adv. 31 346
Singhal G R, Bhavesh K, Kasariya A R, Sharma and Singh R P 2011 J. Nanopart. Res. 13 2981
Kim J S, Kuk E, Yu K N, Kim J H, Park S J, Lee H J, Kim S H, Park Y K, Park Y H, Hwang C Y, Kim Y K, Lee Y S, Jeong D H and Cho M H 2007 Nanomedicine: Nanotechnol. Biol. Med. 3 95
Rai M, Yadav A and Gade A 2009 Biotechnol. Adv. 27 76
Chen S, Wu G and Zeng H 2005 Carbohydr. Polym. 60 33
Castellano J J, Shafii S M, Ko F, Donate G, Wright T E, Mannari R J, Payne W G, Smith D J and Robson M C 2007 Int. Wound J. 4 114
Jung R, Kim Y, Kim H S and Jin H J 2009 J. Biomater. Sci. Polym. Ed. 20 311
Yu D G 2007 Colloids Surf. B: Biointerfaces 59 171
Liu Y C and Lin L N 2004 Electrochem. Commun. 6 1163
Mallick K, Witcombb M J and Scurrella M S 2005 Mater. Chem. Phys. 90 221
Nayak R R, Pradhan N, Behera D, Pradhan K M, Mishra S, Sukla L B and Mishra B K 2011 J. Nanopart. Res. 13 3129
Pattabi M and Uchil J 2000 Sol. Energy Mater. Sol. Cell 63 309
Lin C Y C, Jacob D J, Munger J W and Fiore A M 2000 Geophys. Res. Lett. 27 3465
Jae Y S and Beom S K 2009 Bioprocess. Biosyst. Eng. 32 79
Hutchison J E 2008 ACS Nano. 2 395
Udayasoorian C, Kumar R V and Jayabalakrishnan M 2011 J. Nanomater. Biostruct. 6 537
Visconti A, Minervini F, Lucivera G and Gambatesa V 1991 Mycopathology 113 181
Ingle A, Gade A, Pierrat S, Sonnichsen C and Rai M 2008 Curr. Nanosci. 4 141
Mock J J, Barbic M, Smith D R, Shultz D A and Shultz S 2002 J. Chem. Phys. 116 6755
Prabhu S and Poulose E K 2012 Nano Lett. 2 1
Kaler A, Jain S and Banerjee U C 2013 BioMed. Res. Int. 2013 8
Shankar S, Ahmad A and Sastry M 2003 Biotechnol. Prog. 19 1627
Venkata Subbaiah K P and Savithramma N 2013 Iajpr 3 1285
Sondi I and Salopek-Sondi B 2004 J. Colloid Interface Sci. 275 177
Morones J R, Elechiguerra J L, Camacho A, Holt K, Kouri J B, Ramirez J T and Yacaman M J 2005 J. Nanotechnol. 16 2346
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We are thankful to the Director, and Joint Director, DRDO-BU-CLS for providing necessary facilities to conduct the studies.
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PREMASUDHA, P., VENKATARAMANA, M., ABIRAMI, M. et al. Biological synthesis and characterization of silver nanoparticles using Eclipta alba leaf extract and evaluation of its cytotoxic and antimicrobial potential. Bull Mater Sci 38, 965–973 (2015). https://doi.org/10.1007/s12034-015-0945-5
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DOI: https://doi.org/10.1007/s12034-015-0945-5