Bulletin of Materials Science

, Volume 35, Issue 3, pp 319–326

PLGA 50:50 nanoparticles of paclitaxel: Development, in vitro anti-tumor activity in BT-549 cells and in vivo evaluation

  • RANJITH K AVERINENI
  • GOPAL V SHAVI
  • ARAVIND K GURRAM
  • PRAFUL B DESHPANDE
  • KARTHIK ARUMUGAM
  • NASEER MALIYAKKAL
  • SREENIVASA R MEKA
  • UDUPA NAYANABHIRAMA
Article

DOI: 10.1007/s12034-012-0313-7

Cite this article as:
AVERINENI, R.K., SHAVI, G.V., GURRAM, A.K. et al. Bull Mater Sci (2012) 35: 319. doi:10.1007/s12034-012-0313-7

Abstract

Clinical administration of paclitaxel is hindered due to its poor solubility, which necessitates the formulation of novel drug delivery systems to deliver such extreme hydrophobic drug. To formulate nanoparticles which makes suitable to deliver hydrophobic drugs effectively (intravenous) with desired pharmacokinetic profile for breast cancer treatment; in this context in vitro cytotoxic activity was evaluated using BT-549 cell line. PLGA nanoparticles were prepared by emulsion solvent evaporation technique and evaluated for physicochemical parameters, in vitro anti-tumor activity and in vivo pharmacokinetic studies in rats. Particle size obtained in optimized formulation was <200 nm. Encapsulation efficiency was higher at polymer-to-drug ratio of 20:1. In vitro drug release exhibited biphasic pattern with initial burst release followed by slow and continuous release (15 days). In vitro anti-tumor activity of optimized formulation inhibited cell growth for a period of 168 h against BT-549 cells. AUC(0−∞) and t1/2 were found to be higher for nanoparticles with low clearance rate.

Keywords

PLGA 50:50paclitaxelBT-549 cellspharmacokinetics

Copyright information

© Indian Academy of Sciences 2012

Authors and Affiliations

  • RANJITH K AVERINENI
    • 2
  • GOPAL V SHAVI
    • 1
  • ARAVIND K GURRAM
    • 1
  • PRAFUL B DESHPANDE
    • 1
  • KARTHIK ARUMUGAM
    • 1
  • NASEER MALIYAKKAL
    • 1
  • SREENIVASA R MEKA
    • 2
  • UDUPA NAYANABHIRAMA
    • 2
  1. 1.Department of Pharmaceutics, Manipal College of Pharmaceutical SciencesManipal UniversityManipalIndia
  2. 2.Department of Pharmaceutical SciencesSouth Dakota State UniversitySouth DakotaUSA