Molecular Biotechnology

, Volume 56, Issue 7, pp 599–608

Guidelines for the Tetra-Primer ARMS–PCR Technique Development

  • Ruan Felipe Vieira Medrano
  • Camila Andréa de Oliveira
Research

DOI: 10.1007/s12033-014-9734-4

Cite this article as:
Medrano, R.F.V. & de Oliveira, C.A. Mol Biotechnol (2014) 56: 599. doi:10.1007/s12033-014-9734-4

Abstract

The tetra-primer amplification refractory mutation system–polymerase chain (ARMS–PCR) reaction is a simple and economical method to genotype single-nucleotide polymorphisms (SNPs). It uses four primers in a single PCR and is followed just by gel electrophoresis. However, the optimization step can be very hardworking and time-consuming. Hence, we propose to demonstrate and discuss critical steps for its development, in a way to provide useful information. Two SNPs that provided different amplification conditions were selected. DNA extraction methods, annealing temperatures, PCR cycles protocols, reagents, and primers concentration were also analyzed. The use of tetra-primer ARMS–PCR could be impaired for SNPs in DNA regions rich in cytosine and guanine and for samples with DNA not purified. The melting temperature was considered the factor of greater interference. However, small changes in the reagents concentration significantly affect the PCR, especially MgCl2. Balancing the inner primers band is also a key step. So, in order to balance the inner primers band, intensity is important to observe which one has the weakest band and promote its band by increasing its concentration. The use of tetra-primer ARMS–PCR attends the expectations of modern genomic research and allows the study of SNPs in a fast, reliable, and low-cost way.

Keywords

PolymorphismSNPGenotypingOptimizationTetra-primer ARMS–PCR

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Ruan Felipe Vieira Medrano
    • 1
  • Camila Andréa de Oliveira
    • 2
  1. 1.School of BiomedicineCentro Universitário Hermínio Ometto/UNIARARASSão PauloBrazil
  2. 2.Laboratory of Genetics and Molecular Biology, Biomedical Sciences Graduate ProgramCentro Universitário Hermínio Ometto/UNIARARASArarasBrazil