Molecular Biotechnology

, Volume 54, Issue 2, pp 292–303

The Xerophyta viscosa Aldose Reductase (ALDRXV4) Confers Enhanced Drought and Salinity Tolerance to Transgenic Tobacco Plants by Scavenging Methylglyoxal and Reducing the Membrane Damage

Authors

  • Deepak Kumar
    • School of Life SciencesJawaharlal Nehru University
  • Preeti Singh
    • School of Life SciencesJawaharlal Nehru University
  • Mohd Aslam Yusuf
    • School of Life SciencesJawaharlal Nehru University
  • Chandrama Prakash Upadhyaya
    • School of Life SciencesJawaharlal Nehru University
    • Department of BotanyGuru Ghasidas Central University
  • Suchandra Deb Roy
    • School of Life SciencesJawaharlal Nehru University
  • Thomas Hohn
    • Institute of BotanyUniversity of Basel
    • School of Life SciencesJawaharlal Nehru University
Research

DOI: 10.1007/s12033-012-9567-y

Cite this article as:
Kumar, D., Singh, P., Yusuf, M.A. et al. Mol Biotechnol (2013) 54: 292. doi:10.1007/s12033-012-9567-y

Abstract

We report the efficacy of an aldose reductase (ALDRXV4) enzyme from Xerophyta viscosa Baker in enhancing the prospects of plant’s survival under abiotic stress. Transgenic tobacco plants overexpressing ALDRXV4 cDNA showed alleviation of NaCl and mannitol-induced abiotic stress. The transgenic plants survived longer periods of water deficiency and salinity stress and exhibited improved recovery after rehydration as compared to the wild type plants. The increased synthesis of aldose reductase in transgenic plants correlated with reduced methylglyoxal and malondialdehyde accumulation and an elevated level of sorbitol under stress conditions. In addition, the transgenic lines showed better photosynthetic efficiency, less electrolyte damage, greater water retention, higher proline accumulation, and favorable ionic balance under stress conditions. Together, these findings suggest the potential of engineering aldose reductase levels for better performance of crop plants growing under drought and salt stress conditions.

Keywords

Abiotic stressAldose reductaseMethylglyoxalTransgenicsXerophyta viscosa

Abbreviations

FW

Fresh weight

MDA

Malondialdehyde

MG

Methylglyoxal

ROS

Reactive oxygen species

RWC

Relative water content

TW

Turgid weight

WT

Wild type

Copyright information

© Springer Science+Business Media, LLC 2012