Molecular Biotechnology

, Volume 49, Issue 1, pp 65–76

Genetic Relationship of Curcuma Species from Northeast India Using PCR-Based Markers

  • Archana Das
  • Vigya Kesari
  • Vinod M. Satyanarayana
  • Ajay Parida
  • Latha Rangan
Research

DOI: 10.1007/s12033-011-9379-5

Cite this article as:
Das, A., Kesari, V., Satyanarayana, V.M. et al. Mol Biotechnol (2011) 49: 65. doi:10.1007/s12033-011-9379-5

Abstract

Molecular genetic fingerprints of nine Curcuma species from Northeast India were developed using PCR-based markers. The aim involves elucidating there intra- and inter-specific genetic diversity important for utilization, management, and conservation. Twelve random amplified polymorphic DNA (RAPD), 19 Inter simple sequence repeats (ISSRs), and four amplified fragment length polymorphism (AFLP) primers produced 266 polymorphic fragments. ISSR confirmed maximum polymorphism of 98.55% whereas RAPD and AFLP showed 93.22 and 97.27%, respectively. Marker index and polymorphic information content varied in the range of 8.64–48.1, 19.75–48.14, and 25–28 and 0.17–0.48, 0.19–0.48, and 0.25–0.29 for RAPD, ISSR, and AFLP markers, respectively. The average value of number of observed alleles, number of effective alleles, mean Nei’s gene diversity, and Shannon’s information index were 1.93–1.98, 1.37–1.62, 0.23–0.36, and 0.38–0.50, respectively, for three DNA markers used. Dendrograms based on three molecular data using unweighted pair group method with arithmetic mean (UPGMA) was congruent and classified the Curcuma species into two major clusters. Cophenetic correlation coefficient between dendrogram and original similarity matrix were significant for RAPD (r = 0.96), ISSR (r = 0.94), and AFLP (r = 0.97). Clustering was further supported by principle coordinate analysis. High genetic polymorphism documented is significant for conservation and further improvement of Curcuma species.

Keywords

AFLPCurcumaGenetic diversityISSRNortheastRAPD

Abbreviations

AFLP

Amplified fragment length polymorphism

EDTA

Ethylenediaminetetraacetic acid

EtBr

Ethidium bromide

ISSR

Inter simple sequence repeat

PCA

Principle coordinate analysis

PIC

Polymorphic information content

PCR

Polymerase chain reaction

RAPD

Random amplified polymorphic DNA

TE

Tris–EDTA buffer

UPGMA

Unweighted pair group method with arithmetic mean

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Archana Das
    • 1
  • Vigya Kesari
    • 1
  • Vinod M. Satyanarayana
    • 2
  • Ajay Parida
    • 2
  • Latha Rangan
    • 1
  1. 1.Department of BiotechnologyIndian Institute of Technology GuwahatiNorth GuwahatiIndia
  2. 2.M S Swaminathan Research FoundationChennaiIndia