Molecular Biotechnology

, Volume 45, Issue 2, pp 180–186

Cell-Based Assays for High-Throughput Screening

Authors

  • W. Frank An
    • Chemical Biology PlatformThe Broad Institute
    • Chemical Biology PlatformThe Broad Institute
Review

DOI: 10.1007/s12033-010-9251-z

Cite this article as:
An, W.F. & Tolliday, N. Mol Biotechnol (2010) 45: 180. doi:10.1007/s12033-010-9251-z

Abstract

Cell-based assays represent approximately half of all high-throughput screens currently performed. Here, we review in brief the history and status of high-throughput screening (HTS), and summarize some of the challenges and benefits associated with the use of cell-based assays in HTS. Approaches for successful experimental design and execution of cell-based screens are introduced, including strategies for assay development, implementation of primary and secondary screens, and target identification. In doing so, we hope to provide a comprehensive review of the cell-based HTS process and an introduction to the methodologies and techniques used.

Keywords

Assay developmentCell-based assayHigh-content screening (HCS)High-throughput screening (HTS)Small-molecule screeningTarget identification

Copyright information

© Springer Science+Business Media, LLC 2010