Molecular Biotechnology

, Volume 41, Issue 3, pp 201–207

Differential Expression of Epigenetic Modulators During Human Embryonic Stem Cell Differentiation

  • Sharla M. O. Phipps
  • William K. Love
  • Troy E. Mott
  • Lucy G. Andrews
  • Trygve O. Tollefsbol
Research

DOI: 10.1007/s12033-008-9118-8

Cite this article as:
Phipps, S.M.O., Love, W.K., Mott, T.E. et al. Mol Biotechnol (2009) 41: 201. doi:10.1007/s12033-008-9118-8

Abstract

Although the progression of aging and the diseases associated with it are extensively studied, little is known about the initiation of the aging process. Telomerase is down-regulated early in embryonic differentiation, thereby contributing to telomeric attrition and aging. The mechanisms underlying this inhibition remain elusive, but epigenetic studies in differentiating human embryonic stem (hES) cells could give clues about how and when DNA methylation and histone deacetylation work together to contribute to the inactivation of hTERT, the catalytic subunit of telomerase, at the onset of the aging process. We have confirmed the differentiation status of cultured hES colonies with morphological assessment and immunohistochemical stainings for pluripotent stem cells. In hES cells with varying degrees of differentiation, we have shown a stronger association between hES differentiation and expression of the epigenetic regulators DNMT3A and DNMT3B than between genetic modulators of differentiation such as c-MYC. We also propose a new model system for analyses of stem cell regions, which are differentially down-regulating the expression of hTERT and the actions of epigenetic modulators such as the DNMTs and histone methyltransferases.

Keywords

Aging Epigenetics Telomerase Stem cells DNMTs 

Copyright information

© Humana Press 2008

Authors and Affiliations

  • Sharla M. O. Phipps
    • 1
  • William K. Love
    • 1
  • Troy E. Mott
    • 1
  • Lucy G. Andrews
    • 1
  • Trygve O. Tollefsbol
    • 1
    • 2
    • 3
    • 4
  1. 1.Department of BiologyUniversity of Alabama at BirminghamBirminghamUSA
  2. 2.Center for AgingUniversity of Alabama at BirminghamBirminghamUSA
  3. 3.Comprehensive Cancer CenterUniversity of Alabama at BirminghamBirminghamUSA
  4. 4.Clinical Nutrition Research CenterUniversity of Alabama at BirminghamBirminghamUSA

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