Molecular Biotechnology

, Volume 36, Issue 2, pp 142–150

Construction of microarrays for genotyping of DQA using unmodified 45-mer oligonucleotide

Authors

  • Bin-Cheng Yin
    • State Key Laboratory of Bioreactor Engineering, Department of food Engineering and ScienceEast China University of Science & Technology
  • Fei Yue
    • Guangdong Prevention and Treatment Center for Occupational Diseases
    • State Key Laboratory of Bioreactor Engineering, Department of food Engineering and ScienceEast China University of Science & Technology
Original Paper

DOI: 10.1007/s12033-007-0011-7

Cite this article as:
Yin, B., Yue, F. & Ye, B. Mol Biotechnol (2007) 36: 142. doi:10.1007/s12033-007-0011-7

Abstract

The human leukocyte antigen (HLA) class II system is strongly connected to immunological response and its compatibility between tissues is critical in transplantation. The simple robust typing analyses of HLA genes are extremely important. In this paper, we developed an approach based on microarray technology for genotyping of DQA gene. The microarrays were constructed with a total 31 unmodified 45-mer oligonucleotide. The second exon of DQA gene was amplified, and allowed to hybridize with the array. DQA genotypes were assigned by quantitative analysis of the hybridization results. The arrays were evaluated by DQA genotyping of nine reference samples and 120 clinical samples. The results demonstrate that the genotyping accuracy/concordance achieved 97.5% compared with the direct DNA sequencing. Although our methods did not perform high-resolution genotyping, it could be an alternative for serological typing in routine medical practice.

Keywords

Unmodified oligonucleotide Microarray DQA Genotyping

Copyright information

© Humana Press Inc. 2007