Medical Oncology

, 30:516

Association of PYGO2 and EGFR in esophageal squamous cell carcinoma

  • Meysam Moghbeli
  • Mohammad Reza Abbaszadegan
  • Moein Farshchian
  • Mehdi Montazer
  • Reza Raeisossadati
  • Abbas Abdollahi
  • Mohammad Mahdi Forghanifard
Original Paper

DOI: 10.1007/s12032-013-0516-9

Cite this article as:
Moghbeli, M., Abbaszadegan, M.R., Farshchian, M. et al. Med Oncol (2013) 30: 516. doi:10.1007/s12032-013-0516-9

Abstract

Wnt signaling is an important evolutionary conserved pathway that is not only involved in determination of cellular development, self-renewal, and fate, but also has significant roles in tumor development and progression. Deregulation of the Wnt/β-catenin signaling pathway and aberrant expression of its components is commonly observed in solid tumors. Such aberrant regulation of Wnt signaling is commonly related to either malfunction of its components or crosstalk with other cellular processes such as the epidermal growth factor receptor (EGFR) signaling cascade. Therefore, identification of the roles of major involved components may be useful to identify new therapeutic targets for cancer treatment. In this study, we assessed EGFR and PYGO2 mRNA expression in tumors and margin normal tissues from 55 esophageal squamous cell carcinoma (ESCC) patients using real-time qRT-PCR, and evaluated clinicopathology relative to the two genes’ expression levels. Significant PYGO2 and EGFR overexpression was observed in 30.9 % (P = 0.017) and 38.2 % (P = 0.006) of tumors, respectively. PYGO2 and EGFR expression were significantly associated not only with each other (P < 0.001), but also with tumor staging and depth (P < 0.001). Furthermore, PYGO2 expression was significantly correlated with the tumor grade (P = 0.043) and size (P = 0.023). We identify PYGO2 as a new molecular marker of invasive tumors, introducing its probable oncogenic role in ESCC progression and aggressiveness. In line with other reports, we also illustrate the oncogenic function of EGFR in the development of ESCC through advance stages. We also observed a significant correlation between PYGO2 and EGFR in ESCC tumors, which reveals a mutual convergent influence of these factors in tumor progression and development. Considering aberrant expression, mutual positive feedback, and the significant clinical relevance of these genes in ESCC, we introduce them as appropriate therapeutic targets in adjuvant therapy of ESCC.

Keywords

Esophageal squamous cell carcinomaPYGO2EGFRExpressional analysisReal-time PCR

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Meysam Moghbeli
    • 1
  • Mohammad Reza Abbaszadegan
    • 1
    • 2
  • Moein Farshchian
    • 3
  • Mehdi Montazer
    • 4
  • Reza Raeisossadati
    • 5
  • Abbas Abdollahi
    • 6
  • Mohammad Mahdi Forghanifard
    • 5
  1. 1.Division of Human Genetics, Immunology Research Center, Avicenna Research InstituteMashhad University of Medical SciencesMashhadIran
  2. 2.Medical School, Medical Genetics Research CenterMashhad University of Medical SciencesMashhadIran
  3. 3.Department of Biology, Faculty of ScienceFerdowsi University of MashhadMashhadIran
  4. 4.Department of Pathology, Omid HospitalMashhad University of Medical SciencesMashhadIran
  5. 5.Department of Biology, Damghan BranchIslamic Azad UniversityDamghanIran
  6. 6.Department of General Surgery, Ghaem HospitalMashhad University of Medical SciencesMashhadIran