Medical Oncology

, Volume 29, Issue 5, pp 3421–3430

Prediction and identification of B cell epitopes derived from EWS/FLI-l fusion protein of Ewing’s sarcoma

  • Huiwen Liu
  • Lu Huang
  • Jiaquan Luo
  • Wenzhao Chen
  • Zhanmin Zhang
  • Xiang Liao
  • Min Dai
  • Yong Shu
  • Kai Cao
Original Paper

DOI: 10.1007/s12032-012-0243-7

Cite this article as:
Liu, H., Huang, L., Luo, J. et al. Med Oncol (2012) 29: 3421. doi:10.1007/s12032-012-0243-7

Abstract

To predict B cell epitope of Ewing’s sarcoma EWS/FLI-l fusion protein and to analyze its antigenicity and immunogenicity. Comprehensive algorithms were applied to predict the possible B cell epitopes of EWS/FLI-l fusion protein. High-performance liquid chromatography (HPLC) and mass spectrometry (MS) analysis were performed to identify the synthesized epitope peptides, ELISA assays and Western blot to detect the antigenicity, and the immunogenicity of epitope peptides. Three B cell epitopes were screened out, and HPLC and MS analysis confirmed all three synthesized epitope peptides were demandable. ELISA assays verified all three epitope peptides could prime intense antigen–antibody reaction and induce ideal antibody titers after immunization to the New Zealand white rabbit. However, Western blot confirmed that antiserum of one of these epitope peptides could not recognize EWS/FLI-1 protein. Two B cell epitopes, PQDGNKPTETSQPQ and DPDEVARRWGQRKS, derived from EWS/FLI-l protein, are identified to have potential antigenicity and immunogenicity.

Keywords

Ewing’s sarcomaEWS/FLI-l fusion proteinB cell epitopeAntigenicityImmunogenicity

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Huiwen Liu
    • 1
  • Lu Huang
    • 2
  • Jiaquan Luo
    • 1
  • Wenzhao Chen
    • 1
  • Zhanmin Zhang
    • 3
  • Xiang Liao
    • 1
  • Min Dai
    • 1
  • Yong Shu
    • 1
  • Kai Cao
    • 1
  1. 1.Department of OrthopaedicsThe First Affiliated Hospital of Nanchang UniversityNanchangPeople’s Republic of China
  2. 2.Department of Child CareJiangxi Maternal and Child Health HospitalNanchangPeople’s Republic of China
  3. 3.Department of OncologyThe First Affiliated Hospital of Nanchang UniversityNanchangPeople’s Republic of China