Original Paper

Medical Oncology

, Volume 29, Issue 5, pp 3421-3430

First online:

Prediction and identification of B cell epitopes derived from EWS/FLI-l fusion protein of Ewing’s sarcoma

  • Huiwen LiuAffiliated withDepartment of Orthopaedics, The First Affiliated Hospital of Nanchang University
  • , Lu HuangAffiliated withDepartment of Child Care, Jiangxi Maternal and Child Health Hospital
  • , Jiaquan LuoAffiliated withDepartment of Orthopaedics, The First Affiliated Hospital of Nanchang University
  • , Wenzhao ChenAffiliated withDepartment of Orthopaedics, The First Affiliated Hospital of Nanchang University
  • , Zhanmin ZhangAffiliated withDepartment of Oncology, The First Affiliated Hospital of Nanchang University
  • , Xiang LiaoAffiliated withDepartment of Orthopaedics, The First Affiliated Hospital of Nanchang University
  • , Min DaiAffiliated withDepartment of Orthopaedics, The First Affiliated Hospital of Nanchang University
  • , Yong ShuAffiliated withDepartment of Orthopaedics, The First Affiliated Hospital of Nanchang University
  • , Kai CaoAffiliated withDepartment of Orthopaedics, The First Affiliated Hospital of Nanchang University Email author 

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Abstract

To predict B cell epitope of Ewing’s sarcoma EWS/FLI-l fusion protein and to analyze its antigenicity and immunogenicity. Comprehensive algorithms were applied to predict the possible B cell epitopes of EWS/FLI-l fusion protein. High-performance liquid chromatography (HPLC) and mass spectrometry (MS) analysis were performed to identify the synthesized epitope peptides, ELISA assays and Western blot to detect the antigenicity, and the immunogenicity of epitope peptides. Three B cell epitopes were screened out, and HPLC and MS analysis confirmed all three synthesized epitope peptides were demandable. ELISA assays verified all three epitope peptides could prime intense antigen–antibody reaction and induce ideal antibody titers after immunization to the New Zealand white rabbit. However, Western blot confirmed that antiserum of one of these epitope peptides could not recognize EWS/FLI-1 protein. Two B cell epitopes, PQDGNKPTETSQPQ and DPDEVARRWGQRKS, derived from EWS/FLI-l protein, are identified to have potential antigenicity and immunogenicity.

Keywords

Ewing’s sarcoma EWS/FLI-l fusion protein B cell epitope Antigenicity Immunogenicity