Medical Oncology

, Volume 29, Issue 5, pp 3157–3161

Clinicopathological significance of lymphatic vessel density marked by D2-40 and E-cadherin expression in non-small-cell lung cancer

  • Shidai Jin
  • Wei Zhu
  • Qifeng Shi
  • Zhihong Zhang
  • Renhua Guo
Original Paper

DOI: 10.1007/s12032-012-0229-5

Cite this article as:
Jin, S., Zhu, W., Shi, Q. et al. Med Oncol (2012) 29: 3157. doi:10.1007/s12032-012-0229-5

Abstract

The aim of our study is to investigate the relationship between lymphatic vessel density (LVD) marked by D2-40 and E-cadherin expression and the clinicopathological characteristics of patients with non-small-cell lung cancer (NSCLC). Immunohistochemical analysis was used to detect the expression of D2-40 and E-cadherin in 46 archival surgical specimens of human NSCLC and 10 cases of benign pulmonary disease. The LVD positively stained with D2-40 was observed and counted. The LVD was mainly distributed in the tumor borderline. The LVD in NSCLC tissues was significantly higher than in benign lesions (p < 0.001), and the LVD marked by D2-40 was significantly associated with lymph node metastasis (p < 0.001). The reduced expression of E-cadherin was present in NSCLC tissues. The positive rate of E-cadherin expression was negatively correlated with TNM stage (p = 0.027), pathological grade (p = 0.032), and lymph node metastasis (p = 0.014), and not significantly correlated with histologic classification (p = 0.714) in NSCLC tissues. A correlation analysis showed that high LVD marked by D2-40 was correlated with reduced E-cadherin expression in NSCLC tissues (t = 36.476, p < 0.001). The new monoclonal antibody D2-40 can specifically recognize lymphatic endothelial cells in NSCLC tissue. The relationship between LVD marked by D2-40 and the reduced expression of E-cadherin and lymph node metastasis in NSCLC was demonstrated. The detection of D2-40 and E-cadherin may be used as an indicator of lymph node metastasis in NSCLC.

Keywords

Non-small-cell lung cancer D2-40 Lymphatic vessel density E-cadherin 

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Shidai Jin
    • 1
  • Wei Zhu
    • 2
  • Qifeng Shi
    • 2
  • Zhihong Zhang
    • 3
  • Renhua Guo
    • 1
  1. 1.Department of Medical OncologyThe First Affiliated Hospital of Nanjing Medical UniversityNanjingPeople’s Republic of China
  2. 2.Department of PathologyThe People’s Hospital of XishanWuxiPeople’s Republic of China
  3. 3.Department of PathologyThe First Affiliated Hospital of Nanjing Medical UniversityNanjingPeople’s Republic of China

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