Medical Oncology

, Volume 29, Issue 2, pp 899–908

The analysis of CD45 isoforms expression on HBV-specific T cells after liver transplantation

Authors

  • Lelin Pan
    • Key Lab of Multi-Organ Transplantation, Department of Hepatobiliary and Pancreatic SurgeryThe First Affiliated Hospital, School of Medicine, Zhejiang University
  • Wenjin Zhang
    • Key Lab of Multi-Organ Transplantation, Department of Hepatobiliary and Pancreatic SurgeryThe First Affiliated Hospital, School of Medicine, Zhejiang University
  • Jian Zhang
    • Key Lab of Multi-Organ Transplantation, Department of Hepatobiliary and Pancreatic SurgeryThe First Affiliated Hospital, School of Medicine, Zhejiang University
  • Lin Zhou
    • Key Lab of Multi-Organ Transplantation, Department of Hepatobiliary and Pancreatic SurgeryThe First Affiliated Hospital, School of Medicine, Zhejiang University
  • Lanjuan Li
    • Department of Infectious Diseases, The First Affiliated HospitalSchool of Medicine, Zhejiang University
    • Key Lab of Multi-Organ Transplantation, Department of Hepatobiliary and Pancreatic SurgeryThe First Affiliated Hospital, School of Medicine, Zhejiang University
Original Paper

DOI: 10.1007/s12032-011-9833-z

Cite this article as:
Pan, L., Zhang, W., Zhang, J. et al. Med Oncol (2012) 29: 899. doi:10.1007/s12032-011-9833-z
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Abstract

This study aimed to analyze the expression of CD45 on HBV-specific T cells in patients who underwent liver transplantation (LT). We detected the distribution of two CD45 isoforms (CD45RA, CD45RO) and CD27 on peripheral blood lymphocytes including two HBV-specific pentamer cells by flow cytometry. Meanwhile, the levels of tacrolimus, plasma IL-7, and IL-15 were tested by ELISA. CD45RA decreased sharply after LT, whereas the expression of CD45RO+ changed a very little. The proportion of CD4+ CD27+ subsets increased in the span from first 6–36 months after LT. With the up-regulation of IL-7, CD45RA+ CD27+ was reduced and CD45RO+ was increased on HBV-specific T cells in LT group compared with CHB. Therefore, the switch from CD45RA to CD45RO probably depended on IL-7, without being stimulated by hepatitis B virus (HBV) and proliferated by IL-15. IL-7 could aid in maintaining HBV-specific T cells effectively during 3 years.

Keywords

Hepatitis B virusIL-7CD45CD27Liver transplantation

Abbreviations

HBV

Hepatitis B virus

LT

Liver transplantation

TCM cells

Central memory T cells

IL-7

Interleukin-7

IL-15

Interleukin-15

TEM cells

Effector memory T cells

SNP

Single-nucleotide polymorphism

MELD

Model for end-stage liver disease

HBIG

Hepatitis B immunoglobulin

AHB

Acute hepatitis B

CHB

Chronic hepatitis B

STAT

Signal Transducer and Activator of Transcription

HBsAb

Hepatitis B surface antibody

Lamivudine

2′,3′-Dideoxy-3′-thiacytidine

PCR

Polymerase chain reaction

ELISA

Enzyme-linked immunosorbent assay

LOD

Limit of detection

HLA

Human leukocyte antigen

PBMC

Peripheral blood mononuclear cells

FITC

Fluorescein isothiocyanate

PE

Phycoerythrin

APC

Allophyxoxyanin

ALT

Alanine transaminase

CCR

C-C chemokine receptor

TCR

T-cell receptor

IFN-γ

Interferon gamma

Copyright information

© Springer Science+Business Media, LLC 2011