Medical Oncology

, Volume 25, Issue 1, pp 30–39

Epigallocatechin-3-gallate induces apoptosis and cell cycle arrest in HTLV-1-positive and -negative leukemia cells

  • S. Harakeh
  • K. Abu-El-Ardat
  • M. Diab-Assaf
  • A. Niedzwiecki
  • M. El-Sabban
  • M. Rath
Original Paper

DOI: 10.1007/s12032-007-0036-6

Cite this article as:
Harakeh, S., Abu-El-Ardat, K., Diab-Assaf, M. et al. Med Oncol (2008) 25: 30. doi:10.1007/s12032-007-0036-6

Abstract

The objective of this study is to evaluate the efficacy of epigallocatechin gallate against ATL cells. The anti-proliferative and pro-apoptotic effects of EGCG were evaluated in HTLV-1-positive and -negative cells. EGCG exhibited a marked decrease in proliferation of ATL cells at 96 h of treatment. The results indicated that TGF-α was down-regulated whereas levels of TGF-β2 increased. Cell cycle distribution analysis revealed an increase in cells in the pre-G1 phase which was confirmed by ELISA. The results on proteins showed an up-regulation of p53, Bax and p21 protein levels while the levels of Bcl-2α were down-regulated.

Keywords

ApoptosisHuman T-cell Lymphotrophic Virus-1 (HTLV-1)Epigallocatechin-3-gallate (EGCG)p53p21Transforming Growth Factor (TGF)

Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • S. Harakeh
    • 1
  • K. Abu-El-Ardat
    • 1
  • M. Diab-Assaf
    • 1
  • A. Niedzwiecki
    • 2
  • M. El-Sabban
    • 3
  • M. Rath
    • 2
  1. 1.Biology DepartmentAmerican University of BeirutBeirutLebanon
  2. 2.Dr. Rath Research InsituteSanta ClaraUSA
  3. 3.Human Morphology DepartmentAmerican University of BeirutBeirutLebanon