Medical Oncology

, Volume 24, Issue 4, pp 436–444

Position-dependent expression of GADD45α in rat brain tumours

Authors

  • Antonio Brú
    • Department of Applied Mathematics, Faculty of MathematicsComplutense University
  • Carlos del Fresno
    • Research UnitFundación para la Investigación Hospital La Paz
  • Alessandra Soares-Schanoski
    • Research UnitFundación para la Investigación Hospital La Paz
  • Sonia Albertos
    • Gastroenterology UnitInstituto Nacional de Salud Carlos III, Hospital Carlos III
  • Isabel Brú
    • Centro de Salud La Estación
  • Amelia Porres
    • Clinical Biochemistry UnitFundación Jímenez Díaz
  • Eduardo Rollán-Landeras
    • Faculty of VeterinaryComplutense University
  • Ana Dopazo
    • National Center of Cardiovascular Research
  • David Casero
    • Department of Applied Mathematics, Faculty of MathematicsComplutense University
  • Vanesa Gómez-Piña
    • Research UnitFundación para la Investigación Hospital La Paz
  • Lourdes García
    • Research UnitFundación para la Investigación Hospital La Paz
  • Francisco Arnalich
    • Research UnitLa Paz Hospital
  • Rebeca Alvarez
    • National Center of Cardiovascular Research
  • Alexandro Rodríguez-Rojas
    • Research UnitFundación para la Investigación Hospital La Paz
  • Pablo Fuentes-Prior
    • Cardiovascular Research Center, CSIC-ICCC
    • Research UnitFundación para la Investigación Hospital La Paz
Original Paper

DOI: 10.1007/s12032-007-0025-9

Cite this article as:
Brú, A., del Fresno, C., Soares-Schanoski, A. et al. Med Oncol (2007) 24: 436. doi:10.1007/s12032-007-0025-9

Abstract

Although the complex and multifactorial process of tumour growth has been extensively studied for decades, our understanding of the fundamental relationship between tumour growth dynamics and genetic expression profile remains incomplete. Recent studies of tumour dynamics indicate that gene expression in solid tumours would depend on the distance from the centre of the tumour. Since tumour proliferative activity is mainly localised to its external zone, and taking into account that generation and expansion of genetic mutations depend on the number of cell divisions, important differences in gene expression between central and peripheral sections of the same tumour are to be expected. Here, we have studied variations in the genetic expression profile between peripheral and internal samples of the same brain tumour. We have carried out microarray analysis of mRNA expression, and found a differential profile of genetic expression between the two cell subsets. In particular, one major nuclear protein that regulates cell responses to DNA-damaging and stress signals, GADD45α, was expressed at much lower levels in the peripheral zone, as compared to tumour core samples. These differences in GADD45α mRNA transcription levels have been confirmed by quantitative analysis via real time PCR, and protein levels of GADD45α also exhibit the same pattern of differential expression. Our findings suggest that GADD45α might play a major role in the regulation of brain tumour invasive potential.

Keywords

Tumour growthGADD45αp21

Abbreviation

GADD45α

Growth arrest and DNA-damage-inducible gene 45α

Copyright information

© Humana Press Inc. 2007