Journal of Molecular Neuroscience

, Volume 49, Issue 2, pp 277–288

Muscle-Directed Anti-Aβ Single-Chain Antibody Delivery via AAV1 Reduces Cerebral Aβ Load in an Alzheimer’s Disease Mouse Model

  • Junling Yang
  • Abhinandan Pattanayak
  • Min Song
  • Jinghong Kou
  • Hiroaki Taguchi
  • Sudhir Paul
  • Selvarangan Ponnazhagan
  • Robert Lalonde
  • Ken-ichiro Fukuchi
Article

DOI: 10.1007/s12031-012-9877-3

Cite this article as:
Yang, J., Pattanayak, A., Song, M. et al. J Mol Neurosci (2013) 49: 277. doi:10.1007/s12031-012-9877-3

Abstract

We previously reported that anti-amyloid-beta (Aβ) single-chain antibody (scFv59) brain delivery via recombinant adeno-associated virus (rAAV) was effective in reducing cerebral Aβ load in an Alzheimer’s disease (AD) mouse model without inducing inflammation. Here, we investigated the prophylactic effects and mechanism of a muscle-directed gene therapy modality in an AD mouse model. We injected rAAV serotype 1 encoding scFv59 into the right thigh muscles of 3-month-old mice. Nine months later, high levels of scFv59 expression were confirmed in the thigh muscles by both immunoblotting and immunohistochemistry. As controls, model mice were similarly injected with rAAV1 encoding antihuman immunodeficiency virus Gag antibody (scFvGag). AAV1-mediated scFv59 gene delivery was effective in decreasing Aβ deposits in the brain. Compared with the scFvGag group, levels of Aβ in cerebrospinal fluid (CSF) decreased significantly while Aβ in serum tended to increase in the scFv59 group. AAV1-mediated scFv59 gene delivery may alter the equilibrium of Aβ between the blood and brain, resulting in an increased efflux of Aβ from the brain owing to antibody-mediated sequestration/clearance of peripheral Aβ. Our results suggest that muscle-directed scFv59 delivery via rAAV1 may be a prophylactic option for AD and that levels of CSF Aβ may be used to evaluate the efficacy of anti-Aβ immunotherapy.

Keywords

Single-chain antibody Adeno-associated virus Amyloid Alzheimer’s disease Cerebrospinal fluid 

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Junling Yang
    • 1
  • Abhinandan Pattanayak
    • 1
  • Min Song
    • 1
    • 2
  • Jinghong Kou
    • 1
  • Hiroaki Taguchi
    • 3
  • Sudhir Paul
    • 3
  • Selvarangan Ponnazhagan
    • 4
  • Robert Lalonde
    • 5
  • Ken-ichiro Fukuchi
    • 1
  1. 1.Department of Cancer Biology and PharmacologyUniversity of Illinois College of Medicine at PeoriaPeoriaUSA
  2. 2.Department of Neurology, College of MedicineJiuJiang UniversityJiangxiPeople’s Republic of China
  3. 3.Chemical Immunology Research Center, Department of Pathology and Laboratory MedicineUniversity of Texas Houston Medical SchoolHoustonUSA
  4. 4.Pathology, Schools of Medicine and DentistryUniversity of Alabama at BirminghamBirminghamUSA
  5. 5.Department Psychology, ICONES LaboratoryUniversity of RouenMont-Saint-AignanFrance

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