Journal of Molecular Neuroscience

, Volume 49, Issue 3, pp 491–498

Transcription Initiation Factor IIB Involves in Schwann Cell Differentiation after Rat Sciatic Nerve Crush

  • Jiao Yang
  • Jianhua Cao
  • Youhua Wang
  • Jian Xu
  • Zhengming Zhou
  • Xingxing Gu
  • Xiaojuan Liu
  • Hai Wen
  • Hao Wu
  • Chun Cheng
Article

DOI: 10.1007/s12031-012-9865-7

Cite this article as:
Yang, J., Cao, J., Wang, Y. et al. J Mol Neurosci (2013) 49: 491. doi:10.1007/s12031-012-9865-7

Abstract

Transcription Initiation Factor IIB (TFIIB), as a general transcription factor, plays an essential role in preinitiation complex assembly and transcription initiation by recruiting RNA polymerase II to the promoter. However, its distribution and function in peripheral system lesion and repair were still unknown. Here, we investigated the spatiotemporal expression of TFIIB in an acute sciatic nerve crush model in adult rats. Western blot analysis revealed that TFIIB was expressed in normal sciatic nerve. It gradually increased, reached a peak at the seventh day after crush, and then returned to the normal level at 4 weeks. We observed that TFIIB expressed mainly increased in Schwann cells and co-localized with Oct-6. In vitro, we induced Schwann cell differentiation with cyclic adenosine monophosphate (cAMP) and found that TFIIB expression was increased in the differentiated process. TFIIB-specific siRNA inhibited cAMP-induced Schwann cell morphological change and the expression of P0. Collectively, we hypothesized peripheral nerve crush-induced upregulation of TFIIB in the sciatic nerve was associated with Schwann cell differentiation.

Keywords

Sciatic nerve crushSchwann cellsTFIIBDifferentiation

Abbreviations

TFIIB

Transcription Initiation Factor IIB

SCs

Schwann cells

TFIID

Transcription Initiation Factor IID

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

PNS

Peripheral nervous system

SNC

Sciatic nerve crush

cAMP

Cyclic adenosine monophosphate

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Jiao Yang
    • 1
  • Jianhua Cao
    • 2
  • Youhua Wang
    • 3
  • Jian Xu
    • 2
  • Zhengming Zhou
    • 3
  • Xingxing Gu
    • 4
  • Xiaojuan Liu
    • 5
  • Hai Wen
    • 3
  • Hao Wu
    • 3
  • Chun Cheng
    • 1
  1. 1.Department of Immunology, Medical CollegeNantong UniversityNantongPeople’s Republic of China
  2. 2.Department of OrthopaedicsAffiliated Mental Health Center of Nantong UniversityNantongPeople’s Republic of China
  3. 3.Department of OrthopaedicsAffiliated Hospital of Nantong UniversityNantongPeople’s Republic of China
  4. 4.The Jiangsu Province Key Laboratory of NeuroregenerationNantongPeople’s Republic of China
  5. 5.Department of Pathogen Biology, Medical CollegeNantong UniversityNantongPeople’s Republic of China
  6. 6.Department of OrthopaedicsAffiliated Hospital of Nantong UniversityNantongPeople’s Republic of China