Journal of Molecular Neuroscience

, Volume 48, Issue 1, pp 53–65

Mitogen-Activated Protein Kinase Pathways are Involved in the Upregulation of Calcitonin Gene-Related Peptide of Rat Trigeminal Ganglion After Organ Culture

Article

DOI: 10.1007/s12031-012-9772-y

Cite this article as:
Lei, L., Yuan, X., Wang, S. et al. J Mol Neurosci (2012) 48: 53. doi:10.1007/s12031-012-9772-y

Abstract

The trigeminal ganglion (TG) can express and release calcitonin gene-related peptide (CGRP), an important neuropeptide that plays a crucial role in migraine attack and cluster headache. Activation of rat TG increases CGRP expression. However, the regulatory mechanism of CGRP expression in TG neurons remains to be explored. This study aims to evaluate the involvement of mitogen-activated protein kinase (MAPK) pathways in CGRP upregulation after rat TG organ culture. Rat TG was cultured alone for 24 h or cultured in combination with MAPK inhibitors, tumor necrosis factor α (TNF-α), or interleukin 1β (IL-1β) for 24 h. CGRP protein was determined using immunohistochemistry. The mRNA levels of CGRP, TNF-α, and IL-1β were analyzed through real-time quantitative polymerase chain reaction. MAPK phosphorylation was detected via western blot. After rat TG organ culture, the expressions of CGRP, TNF-α, and IL-1β were upregulated at 24 h. The phosphorylation of extracellular signal-regulated kinases (ERK1/2), P38, and c-jun N-terminal kinases (JNK) significantly increased at 30 min compared with fresh rat TG. In addition, both CGRP expression and phosphorylation of ERK1/2, P38, and JNK were enhanced obviously after rat TG treatment with TNF-α or IL-1β compared with fresh rat TG. However, they decreased markedly after rat TG pretreatment with PD98059 (ERK1/2 inhibitor), SB203580 (P38 inhibitor), or SP600125 (JNK inhibitor) compared with rat TG co-culture with TNF-α or IL-1β. In conclusion, the elevated CGRP expression after rat TG organ culture can be regulated via MAPK pathways. The findings provide insight into the molecular mechanisms and experimental evidence for therapeutic targets of migraine.

Keywords

ERK1/2P38JNKNeuronsTNF-αIL-1β

Abbreviations

CGRP

Calcitonin gene-related peptide

ERK1/2

Extracellular signal-regulated kinases 1/2

IL-1β

Interleukin 1β

JNK

c-Jun N-terminal kinases

MAPK

Mitogen-activated protein kinases

P38

P38 protein

TG

Trigeminal ganglion

TNF-α

Tumor necrosis factor α

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Genetics and Molecular Biology, Key Laboratory of Environment and Genes Related to Diseases, Ministry of EducationXi’an Jiaotong University College of MedicineXi’anPeople’s Republic of China
  2. 2.Department of NeurologyThe First Affiliated Hospital of Xi’an Jiaotong University College of MedicineXi’anPeople’s Republic of China
  3. 3.Department of Epidemiology and Medical Statistics, School of Public HealthXi’an Jiaotong University College of MedicineXi’anPeople’s Republic of China