Journal of Molecular Neuroscience

, Volume 46, Issue 1, pp 33–39

Activity-Dependent Neuroprotective Protein Modulates Its Own Gene Expression

  • Moutasem S. Aboonq
  • Sylvia A. Vasiliou
  • Kate Haddley
  • John P. Quinn
  • Vivien J. Bubb
Article

DOI: 10.1007/s12031-011-9562-y

Cite this article as:
Aboonq, M.S., Vasiliou, S.A., Haddley, K. et al. J Mol Neurosci (2012) 46: 33. doi:10.1007/s12031-011-9562-y

Abstract

We investigated whether activity-dependent neuroprotective protein (ADNP) could autoregulate its own expression. Both the endogenous ADNP gene and reporter gene constructs were analysed in response to overexpression of ADNP, supplied either as wild-type ADNP or a mutant form lacking the NAP motif, a motif which has neuroprotective properties. Overexpression of these two forms of ADNP resulted in both decreased endogenous ADNP expression and repressed ADNP promoter-directed reporter gene activity. Chromatin immunoprecipitation demonstrated the ability of ADNP to bind to its own promoter which is consistent with its action as a repressor of both promoter-supported and endogenous ADNP expression.

Keywords

ADNP Gene regulation Neuroprotection Transcription 

Abbreviations

PCR

Polymerase chain reaction

bp

Base pair

m

Minutes

s

Seconds

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Moutasem S. Aboonq
    • 1
  • Sylvia A. Vasiliou
    • 1
  • Kate Haddley
    • 1
  • John P. Quinn
    • 1
  • Vivien J. Bubb
    • 1
  1. 1.Institute of Translational MedicineSherrington Building, Ashton Street, University of LiverpoolLiverpoolUK