Activity-Dependent Neuroprotective Protein Modulates Its Own Gene Expression
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- Aboonq, M.S., Vasiliou, S.A., Haddley, K. et al. J Mol Neurosci (2012) 46: 33. doi:10.1007/s12031-011-9562-y
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We investigated whether activity-dependent neuroprotective protein (ADNP) could autoregulate its own expression. Both the endogenous ADNP gene and reporter gene constructs were analysed in response to overexpression of ADNP, supplied either as wild-type ADNP or a mutant form lacking the NAP motif, a motif which has neuroprotective properties. Overexpression of these two forms of ADNP resulted in both decreased endogenous ADNP expression and repressed ADNP promoter-directed reporter gene activity. Chromatin immunoprecipitation demonstrated the ability of ADNP to bind to its own promoter which is consistent with its action as a repressor of both promoter-supported and endogenous ADNP expression.
KeywordsADNP Gene regulation Neuroprotection Transcription
Polymerase chain reaction