Journal of Molecular Neuroscience

, Volume 42, Issue 2, pp 154–161

Gene Network Analysis to Determine the Effects of Antioxidant Treatment in a Rat Model of Neonatal Hypoxic-Ischemic Encephalopathy

Authors

    • Computational Systems Biology Research GroupAdvanced Science Institute
    • CREST, Japan Science and Technology Agency (JST)
  • Yuto Ueda
    • Section of Psychiatry, Department of Clinical Neuroscience, Faculty of MedicineUniversity of Miyazaki
  • Naoki Adati
    • Computational Systems Biology Research GroupAdvanced Science Institute
  • Aya Kitamoto
    • Computational Systems Biology Research GroupAdvanced Science Institute
  • Akira Sato
    • Computational Systems Biology Research GroupAdvanced Science Institute
    • CREST, Japan Science and Technology Agency (JST)
  • Ming-Chih Huang
    • Computational Systems Biology Research GroupAdvanced Science Institute
    • Department of Biological Sciences and TechnologyNational University of Tainan
  • Jesmine Noor
    • Section of Obstetrics and Gynecology, Department of Reproductive and Developmental Medicine, Faculty of MedicineUniversity of Miyazaki
  • Hiroshi Sameshima
    • Section of Obstetrics and Gynecology, Department of Reproductive and Developmental Medicine, Faculty of MedicineUniversity of Miyazaki
  • Tsuyomu Ikenoue
    • Section of Obstetrics and Gynecology, Department of Reproductive and Developmental Medicine, Faculty of MedicineUniversity of Miyazaki
Article

DOI: 10.1007/s12031-010-9337-x

Cite this article as:
Kojima, T., Ueda, Y., Adati, N. et al. J Mol Neurosci (2010) 42: 154. doi:10.1007/s12031-010-9337-x

Abstract

Neonatal hypoxic-ischemic (HI) encephalopathy can lead to severe brain damage, and is a common cause of neurological handicaps in adulthood. HI can be resolved by the administration of an antioxidant such as 3-methyl-1-phenyl-2-pyrazolin-5-one (MCI-186). In the present study, we performed comprehensive gene expression and gene network analyses using a DNA microarray to elucidate the molecular events responsible for the selective vulnerability of neurons in neonatal HI brain insult and to examine the underlying mechanisms of the effect of MCI-186 on the pathophysiological events in this condition. We used the modified Levine method (Rice model), which has been widely used as an animal model of this condition. A large difference in gene expression was observed between the Rice model and the control group. Up- and downregulated genes after the HI brain insult were mainly related to immune responses and cell death, and neuronal activity, respectively. The effect of MCI-186 administration on gene expression was much less than and contrary to that of the HI brain insult, reflecting the protective effect of MCI-186 in HI brain insult.

Keywords

Hypoxic-ischemic encephalopathyAntioxidantGene expressionDNA microarrayGene network

Supplementary material

12031_2010_9337_MOESM1_ESM.xls (64 kb)
Supplementary Table S1RICE cluster0 genes (XLS 64 kb)
12031_2010_9337_MOESM2_ESM.xls (146 kb)
Supplementary Table S2RICE cluster1 genes (XLS 146 kb)
12031_2010_9337_MOESM3_ESM.xls (62 kb)
Supplementary Table S3RICE cluster2 genes (XLS 62 kb)
12031_2010_9337_MOESM4_ESM.xls (21 kb)
Supplementary Table S4MCI-186 upregulated genes (XLS 21 kb)
12031_2010_9337_MOESM5_ESM.xls (16 kb)
Supplementary Table S5MCI-186 downregulated genes (XLS 16 kb)

Copyright information

© Springer Science+Business Media, LLC 2010