Analysis of Olfactory Ensheathing Glia Transplantation-Induced Repair of Spinal Cord Injury by Electrophysiological, Behavioral, and Histochemical Methods in Rats
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
This study examined the efficacy of transplanting olfactory ensheathing glia (OEG) in repairing spinal cord injury (SCI) using behavioral tests, retrograde labeling, as well as somatosensory and motor evoked potentials in rats. One week after surgery, motor function in OEG-treated rats was significantly superior to untreated controls (P < 0.05). Also, we found that up to 8 weeks following surgery to induce SCI, somatosensory and motor evoked potentials were found in the OEG-treated groups, but not in the transplantation and damage control groups. Retrograde labeling from the area distal to the SCI produced a higher number of labeled neurons in the ventrolateral division of red nucleus and motor cortex of OEG-treated rats compared to controls, which showed no retrograde labeling (P < 0.05). We believe that this study has important implications for characterizing the mechanisms of OEG transplantation as a treatment for SCI.
- Barami, K., & Diaz, F. G. (2000). Cellular transplantation and spinal cord injury. Neurosurgery, 47, 691–700. doi:10.1097/00006123-200009000-00033. CrossRef
- Basso, D. M., Beattie, M. S., & Bresnahan, J. C. (1995). A sensitive and reliable locomotor rating scale for open field testing in rats. Journal of Neurotrauma, 12, 1. doi:10.1089/neu.1995.12.1. CrossRef
- Imaizumi, T., Lankford, K. L., & Kocsis, J. D. (2000). Transplantation of olfactory ensheathing cells or Schwann cells restores rapid and secure conduction across the transected spinal cord. Brain Research, 854, 70–78. doi:10.1016/S0006-8993(99)02285-4. CrossRef
- Jiang, S., Wang, J., Khan, M. I., et al. (2003). Enteric glia promote regeneration of transected dorsal root axons into spinal cord of adult rats. Experimental Neurology, 181, 79–83. doi:10.1016/S0014-4886(02)00030-4. CrossRef
- Lemons, M. L., Howland, D. R., & Anderson, D. K. (1999). Chondroitin sulfate proteoglycan immunoreactivity increases following spinal cord injury and transplantation. Journal of Experimental Neurology, 160, 51–65. doi:10.1006/exnr.1999.7184. CrossRef
- Liu, C. S., Wu, C. H., & Hou, M. (2004). The application of somatosensory and motor evoked potential measurements to spinal cord injury. Chinese Journal of Clinical Rehabilitation, 8, 2024–2026.
- Lu, J., Feron, F., Mackay-Sim, A., & Waite, P. M. (2002). Olfactory ensheathing cells promote locomotor recovery after delayed transplantation into transected spinal cord. Brain, 125, 14–21. doi:10.1093/brain/awf014. CrossRef
- Moon, L. D. F., Leasure, J. L., Gage, F. H., & Bunge, M. B. (2006). Motor enrichment sustains hindlimb movement recovered after spinal cord injury and glial transplantation. Restorative Neurology and Neuroscience, 24, 147–161.
- Pearse, D., Sanchez, A. R., Pereira, F. C., et al. (2007). Transplantation of Schwann cells and/or olfactory ensheathing glia into the contused spinal cord: Survival, migration, axon association, and functional recovery. Glia, 55, 967–1000. doi:10.1002/glia.20490. CrossRef
- Ramon-Cueto, A., Cordero, M. I., Santo-Benito, F. F., & Aliva, J. (2000). Functional recovery of paraplegic rats and motor axon regeneration in their spinal cords by olfactory ensheathing glia. Neuron, 25, 425–435. doi:10.1016/S0896-6273(00)80905-8. CrossRef
- Sung, P. (2004). Evoked potential and spinal cord injury. The Anatomical Record, 26, 298–302.
- Analysis of Olfactory Ensheathing Glia Transplantation-Induced Repair of Spinal Cord Injury by Electrophysiological, Behavioral, and Histochemical Methods in Rats
Journal of Molecular Neuroscience
Volume 41, Issue 1 , pp 25-29
- Cover Date
- Print ISSN
- Online ISSN
- Humana Press Inc
- Additional Links
- Olfactory ensheathing glia
- Spinal cord injury
- Industry Sectors
- Author Affiliations
- 1. Department of Trauma of Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, No. 1095 Jie Fang Street, Wuhan, 430030, Hubei, China