, Volume 36, Issue 1-3, pp 125-131
Date: 20 Jun 2008

A Functional Variable Number of Tandem Repeats is Located at the 5′ Flanking Region of the Human Secretin Gene Plays a Downregulatory Role in Expression

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Abstract

Secretin is a peptide hormone playing multiple functions in the brain–gut axis. In this report, we investigated, by promoter analysis, the potential function of the variable of tandem repeats (VNTR), located at the 5′ upstream region of the human secretin gene, and we demonstrated for the first time that this VNTR could downregulate transcription of the human secretin gene in a promoter-specific manner. The efficiency of VNTR in silencing the promoter was found to be directly related the number of repetitive units residing within. We also showed the deoxyribonucleic acid sequence as well as the length polymorphism of the VNTR of 76 Chinese individuals. These results collectively suggest that VNTR could potentially be a functional regulator to control the expression of the human secretin gene in different individuals.

Leo T.O. Lee and Ian P.Y. Lam contribute equally in this work and should be considered as co-first authors.