NanoBiotechnology

, Volume 3, Issue 2, pp 89–95

Mesoporous Silica Nanoparticles for Cancer Therapy: Energy-Dependent Cellular Uptake and Delivery of Paclitaxel to Cancer Cells

  • Jie Lu
  • Monty Liong
  • Sean Sherman
  • Tian Xia
  • Michael Kovochich
  • Andre E. Nel
  • Jeffrey I. Zink
  • Fuyuhiko Tamanoi
Article

DOI: 10.1007/s12030-008-9003-3

Cite this article as:
Lu, J., Liong, M., Sherman, S. et al. Nanobiotechnol (2007) 3: 89. doi:10.1007/s12030-008-9003-3

Abstract

Biocompatible mesoporous silica nanoparticles, containing the fluorescence dye fluorescein isothiocyanate (FITC), provide a promising system to deliver hydrophobic anticancer drugs to cancer cells. In this study, we investigated the mechanism of uptake of fluorescent mesoporous silica nanoparticles (FMSN) by cancer cells. Incubation with FMSN at different temperatures showed that the uptake was higher at 37°C than at 4°C. Metabolic inhibitors impeded uptake of FMSN into cells. The inhibition of FMSN uptake by nocodazole treatment suggests that microtubule functions are required. We also report utilization of mesoporous silica nanoparticles to deliver a hydrophobic anticancer drug paclitaxel to PANC-1 cancer cells and to induce inhibition of proliferation. Mesoporous silica nanoparticles may provide a valuable vehicle to deliver hydrophobic anticancer drugs to human cancer cells.

Keywords

mesoporous silica nanoparticleendocytosishydrophobic drugsdrug deliverycancer

Copyright information

© Humana Press Inc. 2008

Authors and Affiliations

  • Jie Lu
    • 1
    • 4
  • Monty Liong
    • 2
    • 4
  • Sean Sherman
    • 1
    • 4
  • Tian Xia
    • 3
    • 4
  • Michael Kovochich
    • 3
    • 4
  • Andre E. Nel
    • 3
    • 4
  • Jeffrey I. Zink
    • 2
    • 4
  • Fuyuhiko Tamanoi
    • 1
    • 4
    • 5
  1. 1.Department of Microbiology, Immunology and Molecular Genetics, Jonsson Comprehensive Cancer Center, Molecular Biology InstituteUniversity of California Los AngelesLos AngelesUSA
  2. 2.Department of Chemistry and BiochemistryUniversity of California Los AngelesLos AngelesUSA
  3. 3.Department of MedicineUniversity of California Los AngelesLos AngelesUSA
  4. 4.California NanoSystems InstituteUniversity of California Los AngelesLos AngelesUSA
  5. 5.Department of Microbiology, Immunology and Molecular Genetics, California NanoSystems Institute, JCCCUniversity of California Los AngelesLos AngelesUSA