Behavioral abnormalities in female mice following administration of aluminum adjuvants and the human papillomavirus (HPV) vaccine Gardasil

  • Rotem Inbar
  • Ronen Weiss
  • Lucija Tomljenovic
  • Maria-Teresa Arango
  • Yael Deri
  • Christopher A. Shaw
  • Joab Chapman
  • Miri Blank
  • Yehuda Shoenfeld
Environment and Autoimmunity

DOI: 10.1007/s12026-016-8826-6

Cite this article as:
Inbar, R., Weiss, R., Tomljenovic, L. et al. Immunol Res (2016). doi:10.1007/s12026-016-8826-6

Abstract

Vaccine adjuvants and vaccines may induce autoimmune and inflammatory manifestations in susceptible individuals. To date most human vaccine trials utilize aluminum (Al) adjuvants as placebos despite much evidence showing that Al in vaccine-relevant exposures can be toxic to humans and animals. We sought to evaluate the effects of Al adjuvant and the HPV vaccine Gardasil versus the true placebo on behavioral and inflammatory parameters in female mice. Six-week-old C57BL/6 female mice were injected with either, Gardasil, Gardasil + pertussis toxin (Pt), Al hydroxide, or, vehicle control in amounts equivalent to human exposure. At 7.5 months of age, Gardasil and Al-injected mice spent significantly more time floating in the forced swimming test (FST) in comparison with vehicle-injected mice (Al, p = 0.009; Gardasil, p = 0.025; Gardasil + Pt, p = 0.005). The increase in floating time was already highly significant at 4.5 months of age for the Gardasil and Gardasil + Pt group (p ≤ 0.0001). No significant differences were observed in the number of stairs climbed in the staircase test which measures locomotor activity. These results indicate that differences observed in the FST were unlikely due to locomotor dysfunction, but rather due to depression. Moreover, anti-HPV antibodies from the sera of Gardasil and Gardasil + Pt-injected mice showed cross-reactivity with the mouse brain protein extract. Immunohistochemistry analysis revealed microglial activation in the CA1 area of the hippocampus of Gardasil-injected mice. It appears that Gardasil via its Al adjuvant and HPV antigens has the ability to trigger neuroinflammation and autoimmune reactions, further leading to behavioral changes.

Keywords

Gardasil Aluminum ASIA syndrome Autoantibodies Autoimmunity Neuroinflammation 

Abbreviations

Al

Aluminum

ASIA

Autoimmune/autoinflammatory syndrome induced by adjuvants

β2-GPI

β2-Glycoprotein I

FST

Forced swimming test

HPV

Human papilloma virus

Pt

Pertussis toxin

U. S FDA

United States Food and Drug Administration

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Rotem Inbar
    • 1
    • 2
  • Ronen Weiss
    • 3
    • 4
  • Lucija Tomljenovic
    • 1
    • 5
  • Maria-Teresa Arango
    • 1
    • 6
  • Yael Deri
    • 1
  • Christopher A. Shaw
    • 5
  • Joab Chapman
    • 1
    • 7
  • Miri Blank
    • 1
  • Yehuda Shoenfeld
    • 1
    • 8
  1. 1.Zabludowicz Center for Autoimmune Diseases, Sheba Medical Center, Tel Hashomer, Sackler Faculty of MedicineTel-Aviv UniversityIsraelIsrael
  2. 2.Department of Obstetrics and GynecologySheba Medical CenterTel HashomerIsrael
  3. 3.Sagol School of NeuroscienceTel Aviv UniversityRamat AvivIsrael
  4. 4.Department of Physiology and Pharmacology, Sackler Faculty of MedicineTel Aviv UniversityRamat AvivIsrael
  5. 5.Neural Dynamics Research Group, Department of Ophthalmology and Visual SciencesUniversity of British ColumbiaVancouverCanada
  6. 6.Doctoral Program in Biomedical SciencesUniversidad del RosarioBogotaColombia
  7. 7.Department of Neurology and Sagol Neuroscience CenterThe Sheba Medical CenterTel HashomerIsrael
  8. 8.Incumbent of the Laura Schwarz-Kip Chair for Research of Autoimmune Diseases, Sackler Faculty of MedicineTel-Aviv UniversityRamat AvivIsrael