Immunologic Research

, Volume 47, Issue 1, pp 172–178

Transcriptional target-based expression cloning of immunoregulatory molecules

Authors

  • Rebecca L. Lamason
    • Department of Biological Chemistry, Institute for Cell Engineering, and Graduate Program in ImmunologyThe Johns Hopkins University School of Medicine
  • Stefanie M. Lew
    • Department of Biological Chemistry, Institute for Cell Engineering, and Graduate Program in ImmunologyThe Johns Hopkins University School of Medicine
    • Department of Biological Chemistry, Institute for Cell Engineering, and Graduate Program in ImmunologyThe Johns Hopkins University School of Medicine
Article

DOI: 10.1007/s12026-009-8148-z

Cite this article as:
Lamason, R.L., Lew, S.M. & Pomerantz, J.L. Immunol Res (2010) 47: 172. doi:10.1007/s12026-009-8148-z

Abstract

Immunologic research has benefited tremendously from expression-cloning strategies designed to isolate genes responsible for a wide variety of immunomodulatory activities, including cytokines, receptors, signaling proteins, and transcription factors. Here, we discuss the use of expression-cloning strategies that have been modified to detect cDNAs that influence gene expression as assayed by a transcriptional reporter. We summarize our experience with these screens, review important parameters, and discuss potential modifications.

Keywords

Expression-cloningGene discoveryNF-κBHIV-1cDNA library

Copyright information

© Springer Science+Business Media, LLC 2010